Rabbit anti-Human CTTN Polyclonal Antibody | anti-CTTN antibody

CTTN, ID (CTTN, EMS1, Src substrate cortactin, Amplaxin, Oncogene EMS1) (AP)

Cat. Num. AAA6289968

• Gene Names: CTTN; EMS1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CTTN; Polyclonal Antibody; ID (CTTN; EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1) (AP); Oncogene EMS1; anti-CTTN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CTTN antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CTTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-302 amino acids from the Central region of human CTTN.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CTTN Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CTTN antibody detected the CTTN protein (arrow).)

Western Blot (WB)

(Western blot analysis of CTTN (arrow) using rabbit polyclonal CTTN Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CTTN gene.)

Immunohistochemistry (IHC)

(CTTN Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CTTN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-CTTN antibody

Antibodies; Proteins

References

Croucher, D.R., et al. Mol. Cell. Biol. 30(21):5057-5070(2010). Eiseler, T., et al. J. Biol. Chem. 285(24):18672-18683(2010). Xu, X.Z., et al. Mod. Pathol. 23(2):187-196(2010). Saitoh, Y., et al. Int. J. Oncol. 35(6):1277-1288(2009). Hirakawa, H., et al. Int. J. Oncol. 35(6):1271-1276(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 20357552
• NCBI GeneID: 2017
• NCBI Accession #: NP_005222.2
• NCBI GenBank Nucleotide #: NM_005231.3
• UniProt Accession #: Q14247; Q8N707; Q96H99; AAB26248
• Molecular Weight: 57,467 Da
• NCBI Official Full Name: src substrate cortactin isoform a
• NCBI Official Synonym Full Names: cortactin
• NCBI Official Symbol: CTTN
• NCBI Official Synonym Symbols: EMS1
• NCBI Protein Information: src substrate cortactin; amplaxin; 1110020L01Rik; oncogene EMS1; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate)
• UniProt Protein Name: Src substrate cortactin
• Protein Family: CTTNBP2 N-terminal-like protein
• UniProt Gene Name: CTTN
• UniProt Synonym Gene Names: EMS1
• UniProt Entry Name: SRC8_HUMAN

NCBI Description

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]

Uniprot Description

Cortactin: a cytoskeletal protein that that is involved in coordinating actin reorganization during cell movement. Localizes at the leading edge of lamellipodia during cell migration. Its amino-terminal acidic domain associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3. Cortactin may play a role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network. It is degraded in a caspase-dependent manner during apoptosis. It is overexpressed in numerous cancers with poor patient prognosis. It may contribute to tumor cell invasion and metastasis. Two splice variant isoforms have been identified.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Cytoskeletal

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cortical cytoskeleton; voltage-gated potassium channel complex; focal adhesion; dendritic spine; actin filament; coated pit; cell cortex; ruffle; growth cone; cytoskeleton; lamellipodium; cytoplasm; podosome

Molecular Function: protein binding

Biological Process: focal adhesion formation; intracellular protein transport; receptor-mediated endocytosis; positive regulation of actin filament polymerization; actin filament polymerization; actin cytoskeleton reorganization; cell motility involved in cell locomotion; regulation of axon extension; substrate-bound cell migration, cell extension; neurite morphogenesis