Mouse SAP18 Monoclonal Antibody | anti-SAP18 antibody

SAP18 (SIN3-Associated Protein, p18)

Cat. Num. AAA603809

• Gene Names: SAP18; SAP18P; 2HOR0202; MGC27131
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: SAP18; Monoclonal Antibody; SAP18 (SIN3-Associated Protein; p18); Anti -SAP18 (SIN3-Associated Protein; anti-SAP18 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5i114
• Specificity: Recognizes SAP18 (SIN3-Associated Protein, p18)
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.

• Applicable Applications for anti-SAP18 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western blot analysis.; Dilution: ELISA: 1:8,000
• Immunogen: Recombinant human transcriptional repressor SAP18 (18kD)
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SAP18 antibody

SAP18, also known as Sin3A-associated protein, which includes Sin3 (protein with four putative paired amphipathic helix domains), HDCA1 and HDCA2, is component of the histone deacetylase complex that plays an important role in the regulation of eukaryotic gene expression. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. It also has been shown to play a key role in gene-specific recruitment of the HDAC complex by a number of transcription factors including Gli, GAGA, and Bicoid.

Product Categories/Family for anti-SAP18 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 48146187
• NCBI GeneID: 10284
• UniProt Accession #: O00422; Q2TTR4; Q6IAW9; Q8N606; Q9UF14; B2R494
• Molecular Weight: 17,561 Da
• NCBI Official Full Name: SAP18
• NCBI Official Synonym Full Names: Sin3A-associated protein, 18kDa
• NCBI Official Symbol: SAP18
• NCBI Official Synonym Symbols: SAP18P; 2HOR0202; MGC27131
• NCBI Protein Information: histone deacetylase complex subunit SAP18; OTTHUMP00000018107; OTTHUMP00000211540; sin3-associated polypeptide p18; sin3-associated polypeptide, p18; cell growth inhibiting protein 38; cell growth-inhibiting protein 38; 18 kDa Sin3-associated polypeptide; sin3-associated polypeptide, 18 kDa; cell growth-inhibiting gene 38 protein; histone deacetlyase complex subunit SAP18
• UniProt Protein Name: Histone deacetylase complex subunit SAP18
• Protein Family: Zinc finger A20 domain-containing stress-associated protein
• UniProt Gene Name: SAP18
• UniProt Entry Name: SAP18_HUMAN

NCBI Description

Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. A pseudogene has been identified on chromosome 2. [provided by RefSeq]

Uniprot Description

SAP18: Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Belongs to the SAP18 family.

Chromosomal Location of Human Ortholog: 13q12.11

Cellular Component: nucleoplasm; histone deacetylase complex; cytoplasm; nuclear speck

Molecular Function: protein binding; transcription corepressor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; negative regulation of nuclear mRNA splicing, via spliceosome; positive regulation of apoptosis; negative regulation of gene expression, epigenetic; RNA splicing; gene expression; mRNA processing; regulation of gene expression, epigenetic; regulation of alternative nuclear mRNA splicing, via spliceosome