Mouse a-Actin, Skeletal Muscle Monoclonal Antibody | anti-ACTA1 antibody

a-Actin, Skeletal Muscle

Cat. Num. AAA602996

• Reactivity: Bovine, Frog, Guinea Pig, Human, Rabbit, Rat, Sheep
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: a-Actin; Skeletal Muscle; Monoclonal Antibody; Anti -a-Actin; anti-ACTA1 antibody

• Host: Mouse
• Reactivity: Bovine, Frog, Guinea Pig, Human, Rabbit, Rat, Sheep
• Clonality: Monoclonal
• Isotype: IgM,k
• Clone Number: 0.N.4
• Specificity: Recognizes human alpha-skeletal and alpha-cardiac (sarcomeric) actins as well as neoplasms derived from normal tissues (i.e. rhabdomyosarcomas). Shows no crossreaction with smooth muscle actin. Species Crossreactivity: bovine, rabbit, sheep, rat, guinea pig and frog.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, before the addition of glycerol to 40%. Also available without BSA and azide. See A0760-24X.

• Applicable Applications for anti-ACTA1 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Immunohistochemistry.; Dilution: Immunohistochemistry (formalin/paraffin sections): 1:200 for 20 min at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.
• Immunogen: N-terminal decapeptide of alpha skeletal muscle isoform of actin; acetylated at the N-terminus. Cellular Localization: Cytoplasmic. Epitope: N-terminal decapeptide.
• Positive Control: Skeletal or Cardiac muscle, or Rhabdomyosarcoma
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human skeletal muscle stained with MBS602996 using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of skeletal muscle cells.)

Product Categories/Family for anti-ACTA1 antibody

Antibodies; Abs to Actin Related Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 71894831
• NCBI GeneID: 421534
• NCBI Accession #: NP_001026234.1
• NCBI GenBank Nucleotide #: NM_001031063.1
• UniProt Accession #: P68139; P02568; P99020
• Molecular Weight: 42,051 Da
• NCBI Official Full Name: actin, alpha skeletal muscle
• NCBI Official Synonym Full Names: actin, alpha 1, skeletal muscle
• NCBI Official Symbol: ACTA1
• NCBI Protein Information: actin, alpha skeletal muscle; a-actin; alpha-actin-1
• UniProt Protein Name: Actin, alpha skeletal muscle
• UniProt Gene Name: ACTA1
• UniProt Synonym Gene Names: ACTA
• UniProt Entry Name: ACTS_CHICK

Uniprot Description

Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subunit structure: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

Subcellular location: Cytoplasm › cytoskeleton.

Post-translational modification: Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization

By similarity.

Miscellaneous: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Sequence similarities: Belongs to the actin family.