Mouse anti-Human FAM3D Monoclonal Antibody | anti-FAM3D antibody
FAM3D (Family with Sequence Similarity 3 Member D, EF-7)
Gene Names
FAM3D; EF7; OIT1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
FAM3D; Monoclonal Antibody; FAM3D (Family with Sequence Similarity 3 Member D; EF-7); Anti -FAM3D (Family with Sequence Similarity 3 Member D; anti-FAM3D antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7H133
Specificity
Recognizes human FAM3D. Does not crossreact with recombinant human FAM3A, FAM3B, FAM3C or recombinant mouse FAM3D.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Applicable Applications for anti-FAM3D antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot and Direct ELISA.
Dilution: Western Blot: 1-2ug/ml. The detection limit for recombinant human FAM3D is ~50ng/lane under non-reducing and reducing conditions.
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human FAM3D is ~13ng/well.
Dilution: Western Blot: 1-2ug/ml. The detection limit for recombinant human FAM3D is ~50ng/lane under non-reducing and reducing conditions.
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human FAM3D is ~13ng/well.
Immunogen
Recombinant protein corresponding to aa39-224 from human FAM3D, expressed in NSO cells (NP_620160).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-FAM3D antibody
Family with sequence similarity 3, member D (FAM3D), also known as EF-7, belongs to the novel cytokine-like family whose members are secreted four-helix bundle proteins. Human FAM3D is abundantly expressed in placenta as a 224 amino acid (aa) residue precursor with a 25aa signal sequence. The aa sequence of mature human FAM3D is 74% identical to that of mouse FAM3D.
Product Categories/Family for anti-FAM3D antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,963 Da
NCBI Official Full Name
family with sequence similarity 3, member D
NCBI Official Synonym Full Names
family with sequence similarity 3, member D
NCBI Official Symbol
FAM3D
NCBI Official Synonym Symbols
EF7; OIT1
NCBI Protein Information
family with sequence similarity 3, member D; OTTHUMP00000214165; OTTHUMP00000214166; cytokine-like protein EF-7
UniProt Protein Name
Protein FAM3D
Protein Family
UniProt Gene Name
FAM3D
UniProt Entry Name
FAM3D_HUMAN
Uniprot Description
FAM3D: Belongs to the FAM3 family.
Protein type: Cytokine; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3p14.2
Cellular Component: extracellular region
Molecular Function: cytokine activity
Biological Process: negative regulation of insulin secretion
Research Articles on FAM3D
Similar Products
Product Notes
The FAM3D fam3d (Catalog #AAA602129) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FAM3D (Family with Sequence Similarity 3 Member D, EF-7) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FAM3D can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot and Direct ELISA. Dilution: Western Blot: 1-2ug/ml. The detection limit for recombinant human FAM3D is ~50ng/lane under non-reducing and reducing conditions. Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human FAM3D is ~13ng/well. Researchers should empirically determine the suitability of the FAM3D fam3d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAM3D, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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