FAM3D recombinant protein

FAM3D Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120902

• Gene Names: FAM3D; EF7; OIT1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: FAM3D; FAM3D Protein; Human; Recombinant (His Tag); Human FAM3D Protein (His Tag); EF7 Protein; OIT1 Protein; UNQ567/PRO1130 Protein; family with sequence similarity 3 member D; FAM3D recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Phe224

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Tyr 26
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human FAM3D (Q96BQ1-1)(Met 1-Phe 224) was fused with a polyhistidine tag at the C-terminus
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for FAM3D recombinant protein

Background: Family with sequence similarity 3 (FAM3) family is a novel cytokine-like gene family, which has four genes in this family, FAM3A, FAM3B, FAM3C, and FAM3D, each encoding a protein (224-235 amino acids) with a hydrophobic leader sequence. It had indicated that FAM3B/PANDER (pancreatic derived factor) is highly expressed in pancreas, and FAM3A and FAM3C in almost all tissues. FAM3D is abundantly expressed in placenta and weakly expressed in small intestine. Immunohistochemistry showed that FAM3A is expressed prominently in the vascular endothelium, particularly capillaries. FAM3A and FAM3B protein were both localized to the islets of Langerhans of the endocrine pancreas. Recombinant FAM3B protein has delayed effects on beta-cell function. FAM3C is involved in retinal laminar formation processes in vertebrates. NFATC2, SCP2, CACNA1C, TCRA, POLE, and FAM3D, were associated with narcolepsy. Some of these associations were further supported by gene expression analyses and an association study in essential hypersomnia (EHS), CNS hypersonia similar to narcolepsy.

References

Zhu Y, et al. (2002) Cloning, expression, and initial characterization of a novel cytokine-like gene family. Genomics. 80(2): 144-50.Katahira T, et al. (2010) Secreted factor FAM3C (ILEI) is involved in retinal laminar formation. Biochem Biophys Res Commun. 392(3): 301-6.Shimada M, et al. (2010) An approach based on a genome-wide association study reveals candidate loci for narcolepsy. Hum Genet. 128(4): 433-41.

NCBI and Uniprot Product Information

• NCBI GI #: 20270363
• NCBI GeneID: 131177
• NCBI Accession #: NP_620160.1
• NCBI GenBank Nucleotide #: NM_138805.2
• UniProt Accession #: Q96BQ1; Q547G2
• Molecular Weight: 24,963 Da
• NCBI Official Full Name: protein FAM3D
• NCBI Official Synonym Full Names: family with sequence similarity 3, member D
• NCBI Official Symbol: FAM3D
• NCBI Official Synonym Symbols: EF7; OIT1
• NCBI Protein Information: protein FAM3D; cytokine-like protein EF-7
• UniProt Protein Name: Protein FAM3D
• Protein Family: Protein
• UniProt Gene Name: FAM3D
• UniProt Entry Name: FAM3D_HUMAN

Uniprot Description

FAM3D: Belongs to the FAM3 family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3p14.2

Cellular Component: extracellular region

Molecular Function: cytokine activity

Biological Process: negative regulation of insulin secretion