Mouse anti-Human ESAM Monoclonal Antibody | anti-ESAM antibody
ESAM (Endothelial Cell Adhesion Molecule)
Gene Names
ESAM; W117m
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
ESAM; Monoclonal Antibody; ESAM (Endothelial Cell Adhesion Molecule); Anti -ESAM (Endothelial Cell Adhesion Molecule); anti-ESAM antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
7H234
Specificity
Selected for its ability to detect human ESAM in direct ELISAs and Western Blots. In these applications, this antibody shows no crossreactivity with rmESAM.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.
Applicable Applications for anti-ESAM antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
Western Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect human ESAM. Using a colorimetric detection system, the detection limit for rhESAM is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold., Flow Cytometry: This antibody was validated for flow cytometry using HUVECs. Dilute this antibody to 25ug/ml and add 10 L of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. HUVECs were stained with anti-ESAM or isotype control antibody, followed by PE-conjugated anti-mouse antibody., Immunocytochemistry: This antibody was used at a concentration of 10ug/ml to detect ESAM in HUVECs. Cells were fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA. After blocking, cells were incubated with diluted primary antibody followed by NL557-coupled anti-mouse IgG in the dark. Between each step, cells were washed with PBS containing BSA., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human ESAM. The detection limit for rhESAM is approximately 1 ng/well. Optimal dilutions should be determined by each laboratory for each application.
Immunogen
NS0-derived rhESAM
Hybridoma
Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human ESAM (rhESAM; aa30-247; Accession # NP_620411).
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-ESAM antibody
Endothelial cell adhesion molecule (ESAM) is a 55kD type I transmembrane glycoprotein of the JAM family of immunoglobulin superfamily molecules. The 390 aa human ESAM contains a 216 aa extracellular domain (ECD) with a V-type and a C2-type Ig domain. The ECD of human and mouse ESAM share 69% aa identity. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes. ESAM mediates endothelial cell homophilic adhesion and supports neutrophil extravasation through tight junctions.
Product Categories/Family for anti-ESAM antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,176 Da
NCBI Official Full Name
endothelial cell-selective adhesion molecule
NCBI Official Synonym Full Names
endothelial cell adhesion molecule
NCBI Official Symbol
ESAM
NCBI Official Synonym Symbols
W117m
NCBI Protein Information
endothelial cell-selective adhesion molecule; 2310008D05Rik; LP4791 protein; OTTHUMP00000200894; OTTHUMP00000200895; HUEL (C4orf1)-interacting protein
UniProt Protein Name
Endothelial cell-selective adhesion molecule
Protein Family
UniProt Gene Name
ESAM
UniProt Entry Name
ESAM_HUMAN
Uniprot Description
ESAM: Can mediate aggregation most likely through a homophilic molecular interaction.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q24.2
Cellular Component: tight junction; adherens junction; plasma membrane; integral to membrane
Biological Process: cell-cell adhesion; blood coagulation; homophilic cell adhesion; leukocyte migration
Research Articles on ESAM
Similar Products
Product Notes
The ESAM esam (Catalog #AAA601874) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ESAM (Endothelial Cell Adhesion Molecule) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ESAM can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Western Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect human ESAM. Using a colorimetric detection system, the detection limit for rhESAM is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold., Flow Cytometry: This antibody was validated for flow cytometry using HUVECs. Dilute this antibody to 25ug/ml and add 10 L of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. HUVECs were stained with anti-ESAM or isotype control antibody, followed by PE-conjugated anti-mouse antibody., Immunocytochemistry: This antibody was used at a concentration of 10ug/ml to detect ESAM in HUVECs. Cells were fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA. After blocking, cells were incubated with diluted primary antibody followed by NL557-coupled anti-mouse IgG in the dark. Between each step, cells were washed with PBS containing BSA., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human ESAM. The detection limit for rhESAM is approximately 1 ng/well. Optimal dilutions should be determined by each laboratory for each application. Researchers should empirically determine the suitability of the ESAM esam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ESAM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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