Mouse anti-Human Podoplanin, FITC-conjugated Monoclonal Antibody | anti-PDPN antibody

Mouse Anti-Human Podoplanin

Cat. Num. AAA690680

• Gene Names: PDPN; T1A; GP36; GP40; Gp38; OTS8; T1A2; TI1A; T1A-2; AGGRUS; HT1A-1; PA2.26
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein G purified
• Synonyms: Podoplanin; FITC-conjugated; Monoclonal Antibody; Mouse Anti-Human Podoplanin; Anti-human Podoplanin-FITC (#18H5); Glycoprotein 36; GP36; T1-alpha; PA2.26 antigen; anti-PDPN antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 18H5
• Specificity: The unconjugated monoclonal antibody will detect native human Podoplanin in WB and on the surface of human LEC.
• Purity/Purification: Protein G purified
• Form/Format: Liquid
• Concentration: 200 ug/ml (varies by lot)
• Sequence Length: 120

• Applicable Applications for anti-PDPN antibody: Flow Cytometry (FC/FACS)
• Application Notes: The suggested use of the antibody is < = 0.5 ug in 100 ul volume. It is recommended that the conjugate antibody should be titrated for optimal performance for each application.
• Immunogen: MDCK cells stably expressing gp36 (Podoplanin) protein
• Conjugation: FITC
• Preparation: Monoclonal antibodies were produced with the help of BALB/c mice using MDCK cells stably expressing gp36 (Podoplanin) protein as the immunizing antigen.
• Buffer/Stabilizer: PBS; 1 % BSA
• Preservative: 0,02 % sodium azide
• Warnings: Reagents contain sodium azide. Under acidic conditions sodium azide yields hydrazoic acid, this is extremely toxic. Azide compounds should be diluted with running water before discarding. These precautions are recommended to avoid deposits in plumbing where explosive condition may develop.
• Preparation and Storage: Store protected from light at 2-8 degree C. DO NOT FREEZE!

Testing Data

(FACS analysis with primary human dermal lymphatic endothelialcells (HDLEC). Upper panel: no primary antibody; Middle panel: solelyconjugated secondary antibody; Lower panel: FITC-conjugated anti-humanPodoplanin antibody.)

Testing Data

(FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).)

Related Product Information for anti-PDPN antibody

Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential Olinked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.

References

1. Breiteneder-Geleff et al, Am J Pathol 154:385, 1999 2. Kerjaschki et al, Nature Med 12:230, 2006 3. Kriehuber et al, J Exp Med 194:797, 2001 4. Zimmer. et al, Biochem J 341:277, 1999 5. Kato et al, Tumour Biol 26:195, 2005 6. Katsue-Inoue et al, J Biol Chem 282:25993, 2007 7. Kato et al, Oncogene 23:8552, 2004 8. Schacht. et al, Am J Pathol 166:913, 2005 9. Mishima et al, Acta Neuropathol 111:483, 2006

NCBI and Uniprot Product Information

• NCBI GI #: 54792060
• NCBI GeneID: 10630
• NCBI Accession #: NP_001006625.1
• NCBI GenBank Nucleotide #: NM_001006624.1
• UniProt Accession #: Q86YL7; O60836; O95128; Q7L375; Q8NBQ8; Q8NBR3; A9Z1Y2; B2R6J8; E9PB68; F6QWX5
• NCBI Official Full Name: podoplanin isoform c
• NCBI Official Synonym Full Names: podoplanin
• NCBI Official Symbol: PDPN
• NCBI Official Synonym Symbols: T1A; GP36; GP40; Gp38; OTS8; T1A2; TI1A; T1A-2; AGGRUS; HT1A-1; PA2.26
• NCBI Protein Information: podoplanin; T1-alpha; hT1alpha-1; hT1alpha-2; PA2.26 antigen; glycoprotein 36; glycoprotein, 36-KD; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2)
• UniProt Protein Name: Podoplanin
• Protein Family: Podoplanin
• UniProt Gene Name: PDPN
• UniProt Synonym Gene Names: GP36; Gp36; T1A
• UniProt Entry Name: PDPN_HUMAN

NCBI Description

This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. Ref.1 Ref.6 Ref.10 Ref.11 UniProtKB Q62011

Subcellular location: Membrane; Single-pass type I membrane protein

By similarity. Cell projection › filopodium membrane; Single-pass type I membrane protein

By similarity. Cell projection › lamellipodium membrane; Single-pass type I membrane protein

By similarity. Cell projection › microvillus membrane; Single-pass type I membrane protein

By similarity. Cell projection › ruffle membrane; Single-pass type I membrane protein

By similarity. Note: Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles

By similarity. UniProtKB Q64294 UniProtKB Q62011

Tissue specificity: Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas. Ref.2 Ref.6 Ref.10

Post-translational modification: Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity. Ref.2 Ref.6 Ref.11The N-terminus is blocked

By similarity. UniProtKB Q64294

Sequence similarities: Belongs to the podoplanin family.

Sequence caution: The sequence AAM73655.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAO22143.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAC11550.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAC11557.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAG35495.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.