Western Blot (WB)
(The Western Blots of whole yeast protein extracts with a collection of our antibodies. The blot for MBS6013097 is in the sixth lane, and the number indicates the SDS-PAGE molecular weight in kiloDaltons.)
Mouse anti-Yeast Pdi1p Monoclonal Antibody | anti-MPD1 antibody
Pdi1p (Protein Disulfide Isomerase 1p)
Reactivity
Yeast
Applications
Western Blot, Immunofluorescence
Purity
SupernatantSterile-filtered cell culture fluid from an Integra CL-350 biochamber
Synonyms
Pdi1p; Monoclonal Antibody; Pdi1p (Protein Disulfide Isomerase 1p); Anti -Pdi1p (Protein Disulfide Isomerase 1p); anti-MPD1 antibody
Host
Mouse
Reactivity
Yeast
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
13B852
Specificity
Recognizes yeast Pdi1p.
Purity/Purification
Supernatant
Sterile-filtered cell culture fluid from an Integra CL-350 biochamber
Sterile-filtered cell culture fluid from an Integra CL-350 biochamber
Form/Format
Supplied as a liquid in 10mM sodium azide.
Applicable Applications for anti-MPD1 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence and Western Blot.
Dilution: Immunofluorescence: For yeast cells, gives an ER staining pattern, and for this purpose should be used at a dilution of 1:500-1:5000.
Western Blot: 1:5000-1:10,000 (ECL detection)
Dilution: Immunofluorescence: For yeast cells, gives an ER staining pattern, and for this purpose should be used at a dilution of 1:500-1:5000.
Western Blot: 1:5000-1:10,000 (ECL detection)
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(The Western Blots of whole yeast protein extracts with a collection of our antibodies. The blot for MBS6013097 is in the sixth lane, and the number indicates the SDS-PAGE molecular weight in kiloDaltons.)
Western Blot (WB)
(The Western Blots of whole yeast protein extracts with a collection of our antibodies. The blot for MBS6013097 is in the sixth lane, and the number indicates the SDS-PAGE molecular weight in kiloDaltons.)
Related Product Information for anti-MPD1 antibody
Pdi1p is a yeast protein disulfide isomerase. It is a major endoplasmic reticulum protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins. It also has a role in recognizing proteins targeted for ER-associated degradation (ERAD) and is a member of the protein disulfide isomerase family. Proteins in this family also can break disulfide bonds on substrate proteins. The protein is also known as Eps1p.
Product Categories/Family for anti-MPD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,408 Da
NCBI Official Full Name
protein disulfide isomerase MPD1
NCBI Official Symbol
MPD1
NCBI Protein Information
protein disulfide isomerase MPD1
UniProt Protein Name
Protein disulfide-isomerase MPD1
Protein Family
UniProt Gene Name
MPD1
UniProt Entry Name
MPD1_YEAST
Uniprot Description
Function: Participates in the folding of proteins containing disulfide bonds. Ref.6
Catalytic activity: Catalyzes the rearrangement of -S-S- bonds in proteins.
Subunit structure: Interacts with CNE1 and EPS1. Ref.6
Subcellular location: Endoplasmic reticulum lumen
Potential.
Miscellaneous: Present with 830 molecules/cell in log phase SD medium.
Sequence similarities: Belongs to the protein disulfide isomerase family.Contains 1 thioredoxin domain.
Research Articles on MPD1
Similar Products
Product Notes
The MPD1 mpd1 (Catalog #AAA6013097) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Pdi1p (Protein Disulfide Isomerase 1p) reacts with Yeast and may cross-react with other species as described in the data sheet. AAA Biotech's Pdi1p can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence and Western Blot. Dilution: Immunofluorescence: For yeast cells, gives an ER staining pattern, and for this purpose should be used at a dilution of 1:500-1:5000. Western Blot: 1:5000-1:10,000 (ECL detection). Researchers should empirically determine the suitability of the MPD1 mpd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pdi1p, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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