Typical Testing Data/Standard Curve (for reference only)
Human PDAP1 ELISA Kit | PDAP1 elisa kit
Human PDAP1 (Pdgfa Associated Protein 1) ELISA Kit
Gene Names
Pdap1; PAP; PAP1; HASPP28
Reactivity
Human
Synonyms
PDAP1; Human PDAP1 (Pdgfa Associated Protein 1) ELISA Kit; PDAP1 elisa kit
Reactivity
Human
Specificity
This kit recognizes Human PDAP1 in samples. No significant cross-reactivity or interference between Human PDAP1 and analogues was observed.
Sequence Length
180
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.78-50ng/mL
Sensitivity
0.47ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PDAP1 were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PDAP1 were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PDAP1 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PDAP1 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDAP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDAP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDAP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PDAP1. You can calculate the concentration of Human PDAP1 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDAP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDAP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDAP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PDAP1. You can calculate the concentration of Human PDAP1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
20,605 Da
NCBI Official Full Name
Pdap1 protein, partial
NCBI Official Synonym Full Names
PDGFA associated protein 1
NCBI Official Symbol
Pdap1
NCBI Official Synonym Symbols
PAP; PAP1; HASPP28
NCBI Protein Information
28 kDa heat- and acid-stable phosphoprotein; PDGF-associated protein; PDGFA-associated protein 1
UniProt Protein Name
28 kDa heat- and acid-stable phosphoprotein
Protein Family
UniProt Gene Name
Pdap1
UniProt Synonym Gene Names
PAP; PAP1
UniProt Entry Name
HAP28_MOUSE
Uniprot Description
PDAP1: Enhances PDGFA-stimulated cell growth in fibroblasts, but inhibits the mitogenic effect of PDGFB. Belongs to the PDAP1 family.
Cellular Component: extracellular space
Molecular Function: platelet-derived growth factor binding
Biological Process: platelet-derived growth factor receptor signaling pathway
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Product Notes
The Human PDAP1 pdap1 (Catalog #AAA2511204) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2511204 ELISA Kit recognizes Human PDAP1. It is sometimes possible for the material contained within the vial of "PDAP1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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