Rabbit anti-Human PDIA5 Polyclonal Antibody | anti-PDIA5 antibody
PDIA5 (Protein Disulfide-isomerase A5, Protein Disulfide Isomerase-related Protein, PDIR) (HRP)
Gene Names
PDIA5; PDIR
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PDIA5; Polyclonal Antibody; PDIA5 (Protein Disulfide-isomerase A5; Protein Disulfide Isomerase-related Protein; PDIR) (HRP); anti-PDIA5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PDIA5.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-PDIA5 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human PDIA5, aa1-519 (NP_006801.1).
Immunogen Sequence
MARAGPAWLLLAIWVVLPSWLSSAKVSSLIERISDPKDLKKLLRTRNNVLVLYSKSEVAAENHLRLLSTVAQAVKGQGTICWVDCGDAESRKLCKKMKVDLSPKDKKVELFHYQDGAFHTEYNRAVTFKSIVAFLKDPKGPPLWEEDPGAKDVVHLDSEKDFRRLLKKEEKPLLIMFYAPWCSMCKRMMPHFQKAATQLRGHAVLAGMNVYSSEFENIKEEYSVRGFPTICYFEKGRFLFQYDNYGSTAEDIVEWLKNPQPPQPQVPETPWADEGGSVYHLTDEDFDQFVKEHSSVLVMFHAPWCGHCKKMKPEFEKAAEALHGEADSSGVLAAVDATVNKALAERFHISEFPTLKYFKNGEKYAVPVLRTKKKFLEWMQNPEAPPPPEPTWEEQQTSVLHLVGDNFRETLKKKKHTLVMFYAPWCPHCKKVIPHFTATADAFKDDRKIACAAVDCVKDKNQDLCQQEAVKGYPTFHYYHYGKFAEKYDSDRTELGFTNYIRALREGDHERLGKKKEEL
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(PDIA5 rabbit polyclonal antibody. Western Blot analysis of PDIA5 expression in human pancreas.)
Western Blot (WB)
(PDIA5 rabbit polyclonal antibody. Western Blot analysis of PDIA5 expression in HeLa.)
Western Blot (WB)
(Western Blot analysis of PDIA5 expression in transfected 293T cell line by PDIA5 polyclonal antibody. Lane 1: PDIA5 transfected lysate (59.6kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-PDIA5 antibody
PDIR (Protein disulfide isomerase related)are endoplasmic reticulum (ER) resident proteins that catalyzse formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins.
Product Categories/Family for anti-PDIA5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,124 Da
NCBI Official Full Name
protein disulfide-isomerase A5
NCBI Official Synonym Full Names
protein disulfide isomerase family A member 5
NCBI Official Symbol
PDIA5
NCBI Official Synonym Symbols
PDIR
NCBI Protein Information
protein disulfide-isomerase A5
UniProt Protein Name
Protein disulfide-isomerase A5
Protein Family
UniProt Gene Name
PDIA5
UniProt Synonym Gene Names
PDIR
UniProt Entry Name
PDIA5_HUMAN
NCBI Description
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. The N-terminal TRX-like domain is the primary binding site for the major ER chaperone calreticulin and possibly other proteins and substrates as well. Alternative splicing results in multiple protein- and non-protein-coding transcript variants. [provided by RefSeq, Dec 2016]
