Western Blot (WB)
(Western Blot analysis of immunized recombinant protein, using MBS601141.)
Mouse MCAF1 Monoclonal Antibody | anti-Cnot7 antibody
MCAF1 (MBD1-containing Chromatin Associated Factor 1, ATF7IP, p621, Activating Transcription Factor 7 Interacting Protein, hAM, ATFa-associated Modulator)
Gene Names
Cnot7; Caf1; Pop2; AU022737
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
MCAF1; Monoclonal Antibody; MCAF1 (MBD1-containing Chromatin Associated Factor 1; ATF7IP; p621; Activating Transcription Factor 7 Interacting Protein; hAM; ATFa-associated Modulator); Anti -MCAF1 (MBD1-containing Chromatin Associated Factor 1; anti-Cnot7 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C222
Specificity
Recognizes human MCAF1.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Applicable Applications for anti-Cnot7 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Recombinant corresponding to a partial sequence near the N-terminal of human MCAF1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of immunized recombinant protein, using MBS601141.)
Western Blot (WB)
(Western Blot analysis of immunized recombinant protein, using MBS601141.)
Related Product Information for anti-Cnot7 antibody
MCAF1 is a recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. Required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9'. The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation.
Product Categories/Family for anti-Cnot7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
32,799 Da
NCBI Official Full Name
mCAF1 protein
NCBI Official Synonym Full Names
CCR4-NOT transcription complex, subunit 7
NCBI Official Symbol
Cnot7
NCBI Official Synonym Symbols
Caf1; Pop2; AU022737
NCBI Protein Information
CCR4-NOT transcription complex subunit 7; CAF-1; POP2 homolog; OTTMUSP00000031948; OTTMUSP00000031949; OTTMUSP00000031950; OTTMUSP00000032045; CCR4-associated factor 1; catabolite repressor protein (CCR4)-associative factor 1
UniProt Protein Name
Putative uncharacterized protein
Protein Family
UniProt Gene Name
Cnot7
UniProt Entry Name
Q3V231_MOUSE
Uniprot Description
CNOT7: Ubiquitous transcription factor required for a diverse set of processes. It is a component of the CCR4 complex involved in the control of gene expression. Belongs to the CAF1 family.
Protein type: Transcription, coactivator/corepressor; EC 3.1.13.4
Cellular Component: membrane; cytoplasm; nucleus; CCR4-NOT complex
Molecular Function: 3'-5'-exoribonuclease activity; protein binding; nuclease activity; poly(A)-specific ribonuclease activity; nucleic acid binding; exoribonuclease activity; hydrolase activity; RNA binding; metal ion binding; exonuclease activity
Biological Process: deadenylation-dependent decapping; regulation of translation; poly(A) tail shortening; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of cell proliferation; cytoplasmic mRNA processing body assembly; RNA-mediated gene silencing; positive regulation of transcription from RNA polymerase II promoter
Research Articles on Cnot7
Similar Products
Product Notes
The Cnot7 cnot7 (Catalog #AAA601141) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MCAF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the Cnot7 cnot7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCAF1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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