Mouse CK-BB Monoclonal Antibody | anti-CKB antibody

CK-BB, NT (CKBB, Creatine Kinase B-type, B-CK, Creatine Kinase B Chain, CKB)

Cat. Num. AAA6007696

• Reactivity: Human, Mouse, Rabbit, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: CK-BB; Monoclonal Antibody; NT (CKBB; Creatine Kinase B-type; B-CK; Creatine Kinase B Chain; CKB); Anti -CK-BB; anti-CKB antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rabbit, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 13A41
• Specificity: Recognizes human creatine kinase B-type. Species Crossreactivity: rabbit, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

• Applicable Applications for anti-CKB antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry.; Dilution: Western Blot: 1:50 (reduced; size ~43-46kD); Immunohistochemistry (Frozen): fixed 2hr in 2% paraformaldehyde and 0.1 % glutaraldehyde in 0.1M PB; stored until use in 1 % paraformaldehyde; blocking with 1% normal swine serum; Immunohistochemistry (Paraffin): antigen retrieval: 0.5% pepsin in 0.01N HCl for 15min at 37 degree C; endogenous peroxidise blocking by using 3% H2O2 in PBS for 30min; blocking with 1% normal swine serum for 30min.; Purkinje cells serve as positive control and connective tissue as negative control
• Immunogen: Synthetic 17-mer peptide (SNSHNALKLRFPAEDEF) corresponding to aa4-20 from human creatine kinase brain-type, at N-terminal.
• Positive Control: Human Purkinje cells
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-CKB antibody

Human CKB is a protein of 381aa (~45kD), expressed in a number of tissues. CKB is most abundant in adult brain, approx. 5-fold lower in the stomach, 10-fold lower in the heart and barely detectable in liver. In brain, whereas most CKB has been shown to be cytosolic, several of the reactions requiring CKB are membrane-associated. CKB belongs to the creatine kinase (CK) isoenzymes that catalyse the synthesis of phosphocreatine (PCr) and its subsequent use in the regeneration of ATP in cell types where the consumption of ATP is rapid and/or sudden. In the brain the different CK isoforms constitute an energy shuttle wherein ATP produced in the mitochondria is used by a mitochondrial CK [e.g. ubiquitous mitochondrial creatine kinase (uMi-CK)] to generate PCr, which is then transported and used by a cytoplasmic CK [e.g. brain creatine kinase (CKB)] to regenerate ATP at discrete cellular sites of high ATP turnover. CKB appears to have a role in regenerating ATP needed for the transport of ions and neurotransmitters since CKB has been localized to brain synaptic plasma membranes, possibly coupled to Na+/K+-ATPase and acetylcholine receptor-rich membranes. Expression of CKB is developmentally controlled: in rat, brain CKB protein at birth is extremely low and increases 10-fold until week 4. This reflects the many energy-demanding processes in brain during brain development.

Product Categories/Family for anti-CKB antibody

Antibodies; Abs to Enzymes; Creatine Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 126723525
• NCBI GeneID: 100009085
• NCBI Accession #: NP_001075730.1
• NCBI GenBank Nucleotide #: NM_001082261.1
• UniProt Accession #: P00567
• Molecular Weight: 42,663 Da
• NCBI Official Full Name: creatine kinase B-type
• NCBI Official Symbol: CKB
• NCBI Protein Information: creatine kinase B-type; B-CK; creatine kinase B chain
• UniProt Protein Name: Creatine kinase B-type
• Protein Family: Creatine kinase
• UniProt Gene Name: CKB
• UniProt Entry Name: KCRB_RABIT

Uniprot Description

Function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activity: ATP + creatine = ADP + phosphocreatine.

Subunit structure: Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

Subcellular location: Cytoplasm.

Sequence similarities: Belongs to the ATP:guanido phosphotransferase family.Contains 1 phosphagen kinase C-terminal domain.Contains 1 phosphagen kinase N-terminal domain.