Rabbit anti-Human, Mouse CKBB Polyclonal Antibody | anti-CKB antibody

CKBB, NT (Creatine Kinase B-type, Creatine Kinase B Chain, B-CK, CKB)

Cat. Num. AAA630397

• Gene Names: CKB; B-CK; CKBB
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: CKBB; Polyclonal Antibody; NT (Creatine Kinase B-type; Creatine Kinase B Chain; B-CK; CKB); Anti -CKBB; anti-CKB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CKB. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-CKB antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the N-terminal region of human CKB (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-CKB Pab is used in Western blot to detect CKB in mouse colon tissue lysate (Lane 1) and Y79 cell lysate (Lane 2).)

Western Blot (WB)

(Western blot analysis of CKB (arrow) using rabbit polyclonal CKB Antibody (N5) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CKB gene.)

Related Product Information for anti-CKB antibody

Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. The CKB isoform, present in many tissues but especially brain, is a cytoplasmic enzyme involved in energy homeostasis. CKB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. Creatine kinase B-driven energy transfer in the brain is important for habituation and spatial learning behavior, mossy fibre field size and determination of seizure susceptibility. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

Product Categories/Family for anti-CKB antibody

Antibodies; Abs to Enzymes; Creatine Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 21536286
• NCBI GeneID: 1152
• NCBI Accession #: NP_001814.2
• NCBI GenBank Nucleotide #: NM_001823.3
• UniProt Accession #: P12277; Q2LE07; Q6FG40; Q9UC66; A8K236; B2R5R4
• Molecular Weight: 42,644 Da
• NCBI Official Full Name: creatine kinase B-type
• NCBI Official Synonym Full Names: creatine kinase, brain
• NCBI Official Symbol: CKB
• NCBI Official Synonym Symbols: B-CK; CKBB
• NCBI Protein Information: creatine kinase B-type; creatine kinase-B; creatine kinase B chain
• UniProt Protein Name: Creatine kinase B-type
• Protein Family: Creatine kinase
• UniProt Gene Name: CKB
• UniProt Synonym Gene Names: CKBB
• UniProt Entry Name: KCRB_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq]

Uniprot Description

CKB: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; EC 2.7.3.2; Kinase, other

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: mitochondrion; cytosol

Molecular Function: protein binding; creatine kinase activity; ATP binding

Biological Process: substantia nigra development; cellular chloride ion homeostasis; creatine metabolic process; phosphorylation