Testing Data
(Diagram of Domain Structure: Generated from sequence of yeast Nsr1p with the SMART program from EMBL in Heidleberg. The first block from the left is a glutamic acid rich region predicted by SMART to encode a region of a-helical coiled coil, though this is likely to be an artifact of the algorithm used. The blocks marked "RRM" indicate two RRM RNA binding domains, each about 70 amino acids long. RRM stands for "RNA recognition motif" and the domain, found in many eukaryotic proteins which bind single stranded RNAs, is also known as the putative RNA-binding region RNP-1 signature. The final block is a region of low sequence complexity, in this case rich in arginine and glycine residues Scale is number of amino acids.)
Mouse Nsr1p Monoclonal Antibody | anti-NSR1 antibody
Nsr1p
Sterile-filtered cell culture fluid from an Integra CL-350 biochamber
Sterile-filtered cell culture fluid from an Integra CL-350 biochamber
Dilution: Immunofluorescence: For yeast cells, try diluted 1:500-1:5000.
Western Blot: For yeast protein samples (cell and nuclear lysates), try diluted 1:10,000, followed by chemiluminescent detection (ECL). For non-ECL Western detection methods, try diluted 1:1000-1:5000.
Testing Data
(Diagram of Domain Structure: Generated from sequence of yeast Nsr1p with the SMART program from EMBL in Heidleberg. The first block from the left is a glutamic acid rich region predicted by SMART to encode a region of a-helical coiled coil, though this is likely to be an artifact of the algorithm used. The blocks marked "RRM" indicate two RRM RNA binding domains, each about 70 amino acids long. RRM stands for "RNA recognition motif" and the domain, found in many eukaryotic proteins which bind single stranded RNAs, is also known as the putative RNA-binding region RNP-1 signature. The final block is a region of low sequence complexity, in this case rich in arginine and glycine residues Scale is number of amino acids.)
Testing Data
(Diagram of Domain Structure: Generated from sequence of yeast Nsr1p with the SMART program from EMBL in Heidleberg. The first block from the left is a glutamic acid rich region predicted by SMART to encode a region of a-helical coiled coil, though this is likely to be an artifact of the algorithm used. The blocks marked "RRM" indicate two RRM RNA binding domains, each about 70 amino acids long. RRM stands for "RNA recognition motif" and the domain, found in many eukaryotic proteins which bind single stranded RNAs, is also known as the putative RNA-binding region RNP-1 signature. The final block is a region of low sequence complexity, in this case rich in arginine and glycine residues Scale is number of amino acids.)
NCBI and Uniprot Product Information
Uniprot Description
Function: Involved in pre-rRNA processing. Specifically binds nuclear localization sequences. Candidate for a receptor at the nucleus that may be involved in both RNA and protein transport. Binds telomeric sequences of the type (TG[1-3])n in vitro.
Subcellular location: Nucleus › nucleolus Ref.8.
Induction: In response to low temperature (By cold-shock).
Post-translational modification: Methylated by HMT1, forming asymmetric dimethylarginines (DMA) within a domain referred to as an RGG box, made up of repeated Gly-Gly dipeptides interspersed with Arg and aromatic residues. Ref.8
Miscellaneous: Present with 77400 molecules/cell in log phase SD medium.
Sequence similarities: Belongs to the RRM GAR family.Contains 2 RRM (RNA recognition motif) domains.
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Product Notes
The NSR1 nsr1 (Catalog #AAA6005395) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Nsr1p can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence and Western Blot. Dilution: Immunofluorescence: For yeast cells, try diluted 1:500-1:5000. Western Blot: For yeast protein samples (cell and nuclear lysates), try diluted 1:10,000, followed by chemiluminescent detection (ECL). For non-ECL Western detection methods, try diluted 1:1000-1:5000. Researchers should empirically determine the suitability of the NSR1 nsr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nsr1p, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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