Rabbit anti-Human RRBP1 Polyclonal Antibody | anti-RRBP1 antibody

RRBP1, CT (RRBP1, KIAA1398, Ribosome-binding protein 1, 180kD ribosome receptor homolog, ES/130-related protein, Ribosome receptor protein)

Cat. Num. AAA6005375

• Gene Names: RRBP1; RRp; hES; ES130; ES/130
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RRBP1; Polyclonal Antibody; CT (RRBP1; KIAA1398; Ribosome-binding protein 1; 180kD ribosome receptor homolog; ES/130-related protein; Ribosome receptor protein); Anti -RRBP1; anti-RRBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Human RRBP1 at ~152kD
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)

• Applicable Applications for anti-RRBP1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:1000; Optimal dilutions to be determined by the researcher.
• Immunogen: Syntetic peptide corresponding to aa1327-1355, from the C-terminal region of human RRBP1, conjugated to KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RRBP1 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the RRBP1 antibody detected the RRBP1 protein (arrow).)

Related Product Information for anti-RRBP1 antibody

Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants.

Product Categories/Family for anti-RRBP1 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 110611220
• NCBI GeneID: 6238
• NCBI Accession #: NP_004578.2
• NCBI GenBank Nucleotide #: NM_004587.2
• UniProt Accession #: Q9P2E9; O75300; O75301; Q5W165; Q96SB2; Q9BWP1; Q9H476; A2A2S6; A6NCN6
• Molecular Weight: 152,472 Da
• NCBI Official Full Name: ribosome-binding protein 1
• NCBI Official Synonym Full Names: ribosome binding protein 1
• NCBI Official Symbol: RRBP1
• NCBI Official Synonym Symbols: RRp; hES; ES130; ES/130
• NCBI Protein Information: ribosome-binding protein 1; ES/130-related protein; ribosome receptor protein; 180 kDa ribosome receptor homolog; ribosome binding protein 1 homolog 180kDa (dog)
• UniProt Protein Name: Ribosome-binding protein 1
• Protein Family: Ribosome-binding protein
• UniProt Gene Name: RRBP1
• UniProt Synonym Gene Names: KIAA1398; RRp
• UniProt Entry Name: RRBP1_HUMAN

NCBI Description

This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function. The full-length nature of variants which differ in repeat length has not been determined. Pseudogenes of this gene have been identified on chromosomes 3 and 7, and RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome, the result of a mutation in a nearby gene on chromosome 20p12. [provided by RefSeq, Apr 2012]

Uniprot Description

RRBP1: a single-pass type III membrane protein of the rough endoplasmic reticulum membrane. Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. Associated with the unfolded protein response (UPR) and has anti-apoptotic activity. Knockdown of RRBP1 induces ER stress, and is associated with a significant reduction in the expression of glucose-regulated protein 78 (GRP78). GRP78 regulates the UPR, is widely upregulated in cancer, and plays an important role in chemotherapy resistance in some cancers. Upregulated in many cancers: overexpressed in a high proportion of breast carcinoma cases tested, and is highly expressed in lung cancer tissue compared with adjacent normal tissues. Because RRBP1 appears to play a role in tumor cell survival, it may be a candidate for new targeted therapeutics. Three human isoforms are produced by alternative splicing.

Protein type: Translation; Ribosomal; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p12

Cellular Component: membrane; endoplasmic reticulum; integral to endoplasmic reticulum membrane; ribosome

Molecular Function: receptor activity

Biological Process: osteoblast differentiation; protein transport; translation