Rabbit anti-Human RRBP1 Polyclonal Antibody | anti-RRBP1 antibody

RRBP1, CT (RRBP1, KIAA1398, Ribosome-binding protein 1, 180kD ribosome receptor homolog, ES/130-related protein, Ribosome receptor protein) (FITC)

Cat. Num. AAA6344076

• Gene Names: RRBP1; RRp; hES; ES130; ES/130
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RRBP1; Polyclonal Antibody; CT (RRBP1; KIAA1398; Ribosome-binding protein 1; 180kD ribosome receptor homolog; ES/130-related protein; Ribosome receptor protein) (FITC); Ribosome receptor protein; anti-RRBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RRBP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a portion of amino acids within aa1327-1355, from the C-terminal region of human RRBP1, conjugated to KLH (NP_004578.2, Q9P2E9).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RRBP1 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the RRBP1 antibody detected the RRBP1 protein (arrow).)

Related Product Information for anti-RRBP1 antibody

Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants.

Product Categories/Family for anti-RRBP1 antibody

Antibodies; Binding Proteins

References

Ueno, T., et al. J. Biol. Chem. 285(39):29941-29950(2010). Ueno, T., et al. Exp. Cell Res. 316(3):329-340(2010). Krasnov, G.S., et al. Mol. Biol. (Mosk.) 43(2):348-356(2009). Benyamini, P., et al. Mol. Biol. Cell 20(2):732-744(2009). Bai, J.Z., et al. Cell Biol. Int. 32(5):473-483(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 110611220
• NCBI GeneID: 6238
• NCBI Accession #: NP_004578.2
• NCBI GenBank Nucleotide #: NM_004587.2
• UniProt Accession #: Q9P2E9; O75300; O75301; Q5W165; Q96SB2; Q9BWP1; Q9H476; A2A2S6; A6NCN6
• Molecular Weight: 108,632 Da
• NCBI Official Full Name: ribosome-binding protein 1
• NCBI Official Synonym Full Names: ribosome binding protein 1
• NCBI Official Symbol: RRBP1
• NCBI Official Synonym Symbols: RRp; hES; ES130; ES/130
• NCBI Protein Information: ribosome-binding protein 1; 180 kDa ribosome receptor homolog; ES/130-related protein; ribosome binding protein 1 homolog 180kDa (dog); ribosome receptor protein
• UniProt Protein Name: Ribosome-binding protein 1
• Protein Family: Ribosome-binding protein
• UniProt Gene Name: RRBP1
• UniProt Synonym Gene Names: KIAA1398; RRp
• UniProt Entry Name: RRBP1_HUMAN

NCBI Description

This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function. The full-length nature of variants which differ in repeat length has not been determined. Pseudogenes of this gene have been identified on chromosomes 3 and 7, and RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome, the result of a mutation in a nearby gene on chromosome 20p12. [provided by RefSeq, Apr 2012]