Rabbit AMPKa Monoclonal Antibody

AMPKa, phosphorylated (Thr172) (AMP-activated protein kinase alpha)

Cat. Num. AAA600380

• Reactivity: Drosophila, Human, Mouse, Rat
• Applications: Western Blot
• Purity: Supernatant; Supernatant
• Synonyms: AMPKa; Monoclonal Antibody; phosphorylated (Thr172) (AMP-activated protein kinase alpha); Anti -AMPKa; anti-AMPKa antibody

• Host: Rabbit
• Reactivity: Drosophila, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 9B144
• Specificity: Recognizes endogenous human AMPK alpha at ~62kD only when phosphorylated at Thr172. Detects both alpha1 and alpha2 isoforms of the catalytic subunit, but does not detect the regulatory beta or gamma subunits. Species Crossreactivity
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-AMPKa antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:2000
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr172 of human AMPK alpha.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western analysis of extracts from SK-N-MC cells, untreated or AICAR-treated, using MBS600380 or A1475-04 (lower).)

Related Product Information for anti-AMPKa antibody

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPK is a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits, each of which is encoded by two or three distinct genes (a1, 2; b1, 2; g1, 2, 3) (2). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia (1). The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPKa at Thr172 in the activation loop and this phosphorylation is required for AMPK activation (3-5). AMPKa is also phosphorylated at Thr258 and Ser485 (for a1; Ser491 for a2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated (6). The b1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108 and Ser182 (6,7). Phosphorylation at Ser108 of the b1 subunit seems to be required for the activation of AMPK enzyme, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization (7). Several mutations in AMPK gamma subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle (1,2). Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS (1).

Product Categories/Family for anti-AMPKa antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 521773886
• NCBI Official Full Name: AMP-activated serine-threonine protein kinase