Typical Testing Data/Standard Curve (for reference only)
Mouse Neurotensin/neuromedin N ELISA Kit | Nts elisa kit
Mouse Neurotensin/neuromedin N ELISA Kit
Gene Names
Nts; NN; NT; NT/N; NTS1; NMN-125; 5033428E16Rik
Reactivity
Mouse
Synonyms
Neurotensin/neuromedin N; Mouse Neurotensin/neuromedin N ELISA Kit; Nts; Nts elisa kit
Reactivity
Mouse
Specificity
This assay recognizes recombinant and natural MouseNeurotensin/neuromedin N. No significant cross-reactivity or interference was observed.
Sequence Length
169
Assay Type
Competitive
Detection Range
78-5000 pg/mL
Sensitivity
41.2 pg/mL
Intra-Assay CV
<=4.2%
Inter-Assay CV
<=6.4%
Recovery
92%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Nts elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of mouseNeurotensin/neuromedin Nconcentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toNeurotensin/neuromedin N, During the reaction,Neurotensin/neuromedin Nin the sample or standard competes with a fixed amount of biotin-labeledNeurotensin/neuromedin N for sites on a pre-coated Monoclonal antibody specific to Neurotensin/neuromedin N. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Neurotensin/neuromedin N in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toNeurotensin/neuromedin N, During the reaction,Neurotensin/neuromedin Nin the sample or standard competes with a fixed amount of biotin-labeledNeurotensin/neuromedin N for sites on a pre-coated Monoclonal antibody specific to Neurotensin/neuromedin N. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Neurotensin/neuromedin N in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,608 Da
NCBI Official Full Name
neurotensin/neuromedin N
NCBI Official Synonym Full Names
neurotensin
NCBI Official Symbol
Nts
NCBI Official Synonym Symbols
NN; NT; NT/N; NTS1; NMN-125; 5033428E16Rik
NCBI Protein Information
neurotensin/neuromedin N
UniProt Protein Name
Neurotensin/neuromedin N
Protein Family
UniProt Gene Name
Nts
UniProt Synonym Gene Names
NN; NmN; NT
UniProt Entry Name
NEUT_MOUSE
Uniprot Description
NTS: Neurotensin may play an endocrine or paracrine role in the regulation of fat metabolism. It causes contraction of smooth muscle. Belongs to the neurotensin family.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: cell soma; cytoplasmic vesicle; extracellular region; nerve terminal
Molecular Function: neuropeptide hormone activity
Biological Process: regulation of blood vessel size; visual learning
Research Articles on Nts
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Product Notes
The Mouse Nts nts (Catalog #AAA2883062) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2883062 ELISA Kit recognizes Mouse Nts. It is sometimes possible for the material contained within the vial of "Neurotensin/neuromedin N, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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