Rat Heat Shock Cognate Protein 70 Monoclonal Antibody | anti-hspa1l antibody

Heat Shock Cognate Protein 70 (HSC70, HSP73) (Biotin)

Cat. Num. AAA600190

• Gene Names: hspa1l; hsc70; hsc70.I; MGC52655
• Reactivity: Bovine, Canine, Chicken, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Sheep
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Heat Shock Cognate Protein 70; Monoclonal Antibody; Heat Shock Cognate Protein 70 (HSC70; HSP73) (Biotin); Anti -Heat Shock Cognate Protein 70 (HSC70; anti-hspa1l antibody

• Host: Rat
• Reactivity: Bovine, Canine, Chicken, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Sheep
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 6i2
• Specificity: Detects a ~73kD protein, corresponding to the apparent molecular mass of Hsc70 on SDS-PAGE Western Blot, in samples from human, mouse, rat, bovine, canine, chicken, guinea pig, hamster, monkey, porcine, rabbit, sheep and turtle origins.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Conjugated to Biotin.

• Applicable Applications for anti-hspa1l antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in ELISA, Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, FACS (2,10), Immunoelectron Microscopy (9).; Dilution: ELISA: 1:100-1:750 (15,16); Western Blot: 1ug/ml (2,4,11); Immunoprecipitation: 5ug/ml (4,13,14); Immunohistochemistry: 1:200 (3,12); Immunocytochemistry: 5ug/ml (4,11); Electron Microscopy: 1:1000 (9)
• Immunogen: Sodium-arsenite-treated heat-resistant variants of Chinese hamster cells (1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%)aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis using MBS600190:| Lane 1: HSC70/HSP73 (bovine); Lane 2: HSP70/HSP72 (human); Lane 3: 3T3 (heat shocked); Lane 4: PC-12 (heat shocked); Lane 6: GPC-16 (heat shocked); Lane 7: HeLa (heat shocked); Lane 8: Vero (heat shocked).)

Related Product Information for anti-hspa1l antibody

This product is the same antibody as H1831-25A but conjugated to Biotin.

The 70kD heat shock cognate protein, Hsc70 is closely related biochemically and biologically to Hsp70 and is part of the Hsp70 family which contains a number of highly related protein isoforms ranging in size from 66kD to 78kD. These proteins include both cognate members which are found within major intracellular compartments and highly inducible isoforms which appear to be predominantly cytoplasmic or nuclear in distribution (3). Members of the Hsp70 family are molecular chaperones which are involved in many cellular functions such as protein folding, transport, maturation and degradation and they exert their function in an ATP-dependent manner. The molecular chaperones of the Hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (4). Recent data has demonstrated that BAG-1 which possesses a ubiquitin-like domain at its amino terminus modulates the chaperone activity of Hsc70 and Hsp70. These findings reveal a role of BAG-1 as a physical link between the Hsc70/Hsp70 chaperone system and the proteasome (5). There is also experimental data which shows that the ATPase domain and the substrate binding domain of Hsc70 cooperate and form a cochaperone-chaperone complex with the synaptic vesicle cysteine string protein (csp) which is essential for normal neurotransmitter release (6).

Product Categories/Family for anti-hspa1l antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 148222597
• NCBI GeneID: 379760
• NCBI Accession #: NP_001080068.1
• NCBI GenBank Nucleotide #: NM_001086599.1
• NCBI Official Full Name: heat shock 70kDa protein 1-like
• NCBI Official Synonym Full Names: heat shock 70kDa protein 1-like
• NCBI Official Symbol: hspa1l
• NCBI Official Synonym Symbols: hsc70; hsc70.I; MGC52655
• NCBI Protein Information: heat shock 70kDa protein 1-like; heat shock cognate protein 70
• Protein Family: Heat shock 70 kDa protein