Western Blot (WB)
(Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using MUNC-18a (Phospho-Ser313) Antibody. The lane on the right is blocked with the phospho peptide.)
Rabbit MUNC-18a Polyclonal Antibody | anti-MUNC-18a antibody
MUNC-18a (Phospho-Ser313) Antibody
Gene Names
STXBP1; P67; NSEC1; UNC18; RBSEC1; MUNC18-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
MUNC-18a; Polyclonal Antibody; MUNC-18a (Phospho-Ser313) Antibody; N- Sec1; STB1; STXB1; STXBP1; Syntaxin binding protein 1; UNC18A; Unc-18 homolog; Unc-18-1; Unc-18A; p67; rbSec1; anti-MUNC-18a antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
MUNC-18a (Phospho-Ser313) Antibody detects endogenous levels of MUNC-18a only when phosphorylated at Ser313.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
603
Applicable Applications for anti-MUNC-18a antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:1000
ELISA: 1:1000
Target Modification
Phospho
Modification Sites
H:S313; M:S313; R:S313
Immunogen
The antiserum was produced against synthesized peptide derived from human MUNC-18a around the phosphorylation site of Ser313.
Immunogen Range
279-328
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Western Blot (WB)
(Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using MUNC-18a (Phospho-Ser313) Antibody. The lane on the right is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using MUNC-18a (Phospho-Ser313) Antibody. The lane on the right is blocked with the phospho peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67 kDa
NCBI Official Full Name
syntaxin-binding protein 1 isoform b
NCBI Official Synonym Full Names
syntaxin binding protein 1
NCBI Official Symbol
STXBP1
NCBI Official Synonym Symbols
P67; NSEC1; UNC18; RBSEC1; MUNC18-1
NCBI Protein Information
syntaxin-binding protein 1
UniProt Protein Name
Syntaxin-binding protein 1
UniProt Gene Name
STXBP1
UniProt Synonym Gene Names
UNC18A; Unc18-1; Unc-18A
NCBI Description
This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010]
Uniprot Description
May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
Research Articles on MUNC-18a
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Product Notes
The MUNC-18a stxbp1 (Catalog #AAA9502497) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MUNC-18a (Phospho-Ser313) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MUNC-18a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:1000. Researchers should empirically determine the suitability of the MUNC-18a stxbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MUNC-18a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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