Rat anti-Human NFkB, p65 Monoclonal Antibody | anti-NKIRAS2 antibody

NFkB, p65 (Transcription Factor p65, Nuclear Factor NF-kappa-B p65 Subunit, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-cells 3, NFKB3, RELA)

Cat. Num. AAA6001754

• Gene Names: NKIRAS2; KBRAS2; kappaB-Ras2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Synonyms: NFkB; p65; Monoclonal Antibody; p65 (Transcription Factor p65; Nuclear Factor NF-kappa-B p65 Subunit; Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-cells 3; NFKB3; RELA); Anti -NFkB; anti-NKIRAS2 antibody

• Host: Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2A
• Clone Number: 12K142
• Specificity: Recognizes human NFkB p65.
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

• Applicable Applications for anti-NKIRAS2 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry.; Dilution: Flow Cytometry: 2.5ug labels/10e6 (Daudi human Burkitt's lymphoma cell line fixed with paraformaldehyde and permeabilized with methanol)
• Immunogen: Recombinant corresponding to aa1-254 from human RelA expressed in E.coli.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Flow Cytometry (FC/FACS)

(Detection of RelA/NFKB p65 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Human RelA/NFKB p65 Monoclonal Antibody or isotype control antibody open histogram, followed by Phycoerythrin-conjugated Rat IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.)

Related Product Information for anti-NKIRAS2 antibody

RelA p65 is a 65kD member of the NFkB family of nuclear transcription factors. Dimers of p65 with the p50 subunit are the most common form of the NFkB transcription factor, but dimers with itself or other family members are also active. An alternatively spliced isoform that lacks aa222-231 (p65D) does not bind DNA. Over the sequence used as an immunogen, human RelA p65 shares 96% and 98% aa identity with mouse and rat RelA p65, respectively. This portion includes one of eight potential Ser/Thr phosphorylation sites, two acetylation sites, and most of the Rel homology domain that interacts with IkB inhibitors.

Product Categories/Family for anti-NKIRAS2 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 222136632
• NCBI GeneID: 28511
• NCBI Accession #: NP_001138401.1
• NCBI GenBank Nucleotide #: NM_001144929.1
• UniProt Accession #: Q9NYR9; Q6PK52; Q96KC7; Q9NSX1; A6NCZ5; B3KNN0; B4DNM3
• Molecular Weight: 21,508 Da
• NCBI Official Full Name: NF-kappa-B inhibitor-interacting Ras-like protein 2 isoform c
• NCBI Official Synonym Full Names: NFKB inhibitor interacting Ras-like 2
• NCBI Official Symbol: NKIRAS2
• NCBI Official Synonym Symbols: KBRAS2; kappaB-Ras2
• NCBI Protein Information: NF-kappa-B inhibitor-interacting Ras-like protein 2; kappa B-Ras protein 2; I-kappa-B-interacting Ras-like protein 2; NFKB inhibitor interacting Ras-like protein 2
• UniProt Protein Name: NF-kappa-B inhibitor-interacting Ras-like protein 2
• Protein Family: NF-kappa-B inhibitor-interacting Ras-like protein
• UniProt Gene Name: NKIRAS2
• UniProt Synonym Gene Names: KBRAS2; Kappa B-Ras protein 2; KappaB-Ras2
• UniProt Entry Name: KBRS2_HUMAN

Uniprot Description

NKIRAS2: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF- kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB. Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric, Ras; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: membrane; cytoplasm

Molecular Function: GTPase activity; GTP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; metabolic process; small GTPase mediated signal transduction