Rabbit anti-Human ROR1 Polyclonal Antibody | anti-Ror1 antibody

ROR1, NT (ROR1, NTRKR1, Tyrosine-protein kinase transmembrane receptor ROR1, Neurotrophic tyrosine kinase, receptor-related 1)

Cat. Num. AAA6000566

• Gene Names: Ror1; mRor1; Ntrkr1; 2810404D04Rik
• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: ROR1; Polyclonal Antibody; NT (ROR1; NTRKR1; Tyrosine-protein kinase transmembrane receptor ROR1; Neurotrophic tyrosine kinase; receptor-related 1); Anti -ROR1; anti-Ror1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-Ror1 antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot 1:100-500; Flow Cytometry: 1:10-50
• Immunogen: ROR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40~69 amino acids from the N-terminal region of human ROR1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of ROR1 (arrow) using rabbit polyclonal ROR1 Antibody (N-term) . 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected with the ROR1 gene (Lane 2) (Origene Technologies).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H460 cells using ROR1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-Ror1 antibody

ROR1 is a receptor protein tyrosine kinase whose cellular role has not been determined. It is a type I membrane protein and belongs to the ROR subfamily of cell surface receptors. Studies of a similar protein in mouse suggest that this protein may interact with another receptor protein tyrosine kinase and may be involved in skeletal and cardiac development.

Product Categories/Family for anti-Ror1 antibody

Antibodies; Abs to Transmembrane Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4514618
• NCBI GeneID: 26563
• UniProt Accession #: Q9Z139; Q8BG10
• Molecular Weight: 104,088 Da
• NCBI Official Full Name: Ror1
• NCBI Official Synonym Full Names: receptor tyrosine kinase-like orphan receptor 1
• NCBI Official Symbol: Ror1
• NCBI Official Synonym Symbols: mRor1; Ntrkr1; 2810404D04Rik
• NCBI Protein Information: tyrosine-protein kinase transmembrane receptor ROR1; neurotrophic tyrosine kinase, receptor related 1; neurotrophic tyrosine kinase, receptor-related 1
• UniProt Protein Name: Tyrosine-protein kinase transmembrane receptor ROR1
• Protein Family: Inactive tyrosine-protein kinase transmembrane receptor
• UniProt Gene Name: Ror1
• UniProt Synonym Gene Names: Ntrkr1; mROR1
• UniProt Entry Name: ROR1_MOUSE

NCBI Description

This gene encodes a receptor tyrosine kinase that has been implicated in nervous system development, specifically in the maintenance of neural progenitor cell fate, neurite extension and synapse formation. The encoded protein, likely a pseudokinase that lacks catalytic activity, may also regulate adipogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

ROR1: Tyrosine-protein kinase receptor whose role is not yet clear. Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; EC 2.7.10.1; TK group; Ror family

Cellular Component: membrane; integral to plasma membrane; integral to membrane; receptor complex

Molecular Function: transferase activity; Wnt-protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; signal transduction; phosphorylation; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway