Mouse Activity Regulated Cytoskeleton Associated Protein (ARC) RTU ELISA Kit | ARC rtu elisa kit

Mouse Activity Regulated Cytoskeleton Associated Protein (ARC) ELISA Kit

Cat. Num. AAA457748

• Gene Names: Arc; mArc; Arc3.1; C86064; arg3.1
• Reactivity: Mouse
• Synonyms: Activity Regulated Cytoskeleton Associated Protein (ARC); Mouse Activity Regulated Cytoskeleton Associated Protein (ARC) ELISA Kit; Arg3.1; Activity-regulated gene 3.1 protein homolog; ARC rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 396

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ARC rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ARC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ARC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ARC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ARC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 451958117
• NCBI GeneID: 11838
• NCBI Accession #: NP_001263613.1
• NCBI GenBank Nucleotide #: NM_001276684.1
• UniProt Accession #: Q9WV31; Q9ES15
• Molecular Weight: 45,321 Da
• NCBI Official Full Name: activity-regulated cytoskeleton-associated protein
• NCBI Official Synonym Full Names: activity regulated cytoskeletal-associated protein
• NCBI Official Symbol: Arc
• NCBI Official Synonym Symbols: mArc; Arc3.1; C86064; arg3.1
• NCBI Protein Information: activity-regulated cytoskeleton-associated protein
• UniProt Protein Name: Activity-regulated cytoskeleton-associated protein
• Protein Family: AAA ATPase forming ring-shaped complexes
• UniProt Gene Name: Arc
• UniProt Synonym Gene Names: Arg3.1

Uniprot Description

Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system (). ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation (). ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons (). Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory (PubMed:29264923, PubMed:24094104). Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis (PubMed:17088213, PubMed:20211139, PubMed:20228806). Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses (PubMed:23791196). Accumulates at weaker synapses, probably to prevent their undesired enhancement (). This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material (). Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory (PubMed:17088210, PubMed:20228806). Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1 (PubMed:22036569). In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation (PubMed:28783680).