Mouse TLR9 Monoclonal Antibody | anti-TLR9 antibody

TLR9, Human, mAb 5G5, biotinylated

Cat. Num. AAA584874

• Gene Names: TLR9; CD289
• Reactivity: Human, Canine, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
• Synonyms: TLR9; Monoclonal Antibody; Human; mAb 5G5; biotinylated; CD289; TLR9 Human mAb 5G5 Biotin 50ug; anti-TLR9 antibody

• Host: Mouse
• Reactivity: Human, Canine, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Form/Format: 0.5ml (100ug/ml) 0.2um filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
• Sequence Length: 1031

• Applicable Applications for anti-TLR9 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Frozen, Immunoassay (IA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: The typical starting working dilution is 1:50.; IHC-F: 10um sections were fixed with acetone for 10 minutes. PBS washed sections were incubated with 5G5 1:100 in 1% BSA for 30 minutes at RT. (Ref.7); IF: cells were fixed with 2% formalin for 15 minutes at RT and permeabilized with a mAb (4ug/400 ul) containing buffer (PBS, 0.2% BSA, 0.2% saponin)- for 1 hour. (Ref.1); FC: RAW264.7 cells were fixed for 15 minutes with 4% formalin and permeabilized (PBS, 0.5%BSA, 0,5% saponin) at RT. (Ref.1); IHC-P: paraffin embedded tissues 5um;m sections were made. After antigen retrieval (0.01mol/l, pH6 sodium citrate) and quenching of endogenous peroxidase, sections were blocked with 0.5% ovalbumin and 0.1% gelatin for 20 minutes at RT. Sections were incubated with 5G5 for 1 hour at 37 degree C. (Ref.3); W: reduced lysates were resolved by 10% SDS-PAGE and blotted on nitrocellulose. After blocking with 5% skimmed milk TLR9 was detected with 2ug/ml 5G5. (Ref.1)
• Immunogen: Purified-fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc
• Conjugation: Biotin
• Preparation and Storage: Product should be stored at 4 degree C.; Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-TLR9 antibody

The monoclonal antibody 5G5 recognizes human Toll-like receptor 9. Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called 'pathogen-associated molecular patterns'. Activation of TLRs, a family of at least 11 differentmembers that function either as homo- or heterodimers, leads to activation of NFkappaB-dependent and IFNregulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens.
Whereas most TLRs are expressed on the cell surface, TLR9 is expressed intracellularly within one or more endosomal compartments and recognizes nucleic acids. TLR9 detects a rather subtle difference in the DNA of vertebrates compared with that of pathogens. Vertebrate genomic DNAs have mostly methylated CpG dinucleotides where bacterial and viral DNAs have unmethylated CpG dinucleotides. TLR9 undergoes relocation from endoplasmic reticulum to CpG-ODN-containing endosomes. In these endosomes TLR9 becomes a functional receptor after proteolytic cleavage. TLR9 exists as a preformed homodimer and CpG-ODN binding promotes its conformational change, bringing the cytoplasmic TIR-like domains close to each other. This allows a recruitment of the key adapter protein MyD88 which initiates a signalling cascade. The only human immune cell types known to constitutively express TLR9 and to be activated by CpG ODN are pDCs and B cells. TLR9 triggering induces an activation phenotype in the B cells and pDCs, characterized by the expression of costimulatory molecules, resistance to apoptosis, and induces Th1-type immune response profiles.

Product Categories/Family for anti-TLR9 antibody

Infectious diseases

References

1. Ahmad-Nejad, P et al; Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments. Eur J Immunol 2002, 32: 1958
2. Rutz, M et al; Toll-like receptor 9 binds single-stranded CpG-DNA in a sequence- and pHdependent manner. Eur J Immunol 2004, 34: 2541
3. Rumio C et al; Degranulation of Paneth cells via Toll-like receptor 9. Am J Pathol 2004, 165:373
4. Pratesi, G et al; Therapeutic synergism of gemcitabine and CpG-oligodeoxynucleotides in an orthotopicHuman pancreatic carcinoma xenograft. Cancer res 2005, 65: 6388
5. Tokumasa, N et al; Expression of Tyk2 in dendritic cells is required for Il-2, Il-23, and IFNg production and the induction of Th1 cell differentiation. Blood 2007, 110: 5536. Burgener, I et al; Antibodies specific forHuman or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol 2008, 124; 184
7. Machida, H et al; Expression of Toll-like receptor 9 in renal podocytes in childhood-onset active and inactive lupus nephritis. Nephrol Dial Transplant 2010, 25: 2530
8. Zheng, W et al. Distinct host-related dendritic cell responses during the early stage of Plasmodium yoelii infection in susceptible and resistant mice. Parasite immunology 2010, 32: 324

NCBI and Uniprot Product Information

• NCBI GI #: 229473723
• NCBI GeneID: 54106
• NCBI Official Full Name: TLR9, partial
• NCBI Official Synonym Full Names: toll like receptor 9
• NCBI Official Symbol: TLR9
• NCBI Official Synonym Symbols: CD289
• NCBI Protein Information: toll-like receptor 9
• Protein Family: Toll-like receptor

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response. [provided by RefSeq, Aug 2017]