Mouse anti-Human Mannose receptor Monoclonal Antibody | anti-MRC1 antibody
Mannose receptor, Human, mAb 15-2, biotinylated
Gene Names
MRC1; MMR; hMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Synonyms
Mannose receptor; Monoclonal Antibody; Human; mAb 15-2; biotinylated; Machrophage Mannose Receptor 1; MRC1; CLEC13D; MMR; C-type lectin domain family 13 member D; Gene name: MRC1; Mannose receptor Human mAb 15-2 Biotin 50ug; anti-MRC1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Form/Format
0.5ml (100ug/ml) 0.2um filtered protein G purified biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Sequence Length
1456
Applicable Applications for anti-MRC1 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Frozen, Functional Studies (FS), Western Blot (WB)
Application Notes
The typical starting working dilution is 1:50.
IHC-F: Tissue sections were fixed in acetone/chloroform. Sections were preincubated with normal rabbit serum (Ref.3).
FC: Antibody 15-2 stains the extracellular domain of MR.
W: A non-reduced sample treatment and 6% SDS-Page was used. The band size is 175 kDa (Ref.1).
FS: Antibody 15-2 functions as an inhibitor of binding. The antibody was functionally tested by preincubation of immobilized receptor with antibody. This prevented binding of t-PA to immobilized receptor. (Ref.1).
IHC-F: Tissue sections were fixed in acetone/chloroform. Sections were preincubated with normal rabbit serum (Ref.3).
FC: Antibody 15-2 stains the extracellular domain of MR.
W: A non-reduced sample treatment and 6% SDS-Page was used. The band size is 175 kDa (Ref.1).
FS: Antibody 15-2 functions as an inhibitor of binding. The antibody was functionally tested by preincubation of immobilized receptor with antibody. This prevented binding of t-PA to immobilized receptor. (Ref.1).
Immunogen
Purified human mannose receptor from human placental tissue
Conjugation
Biotin
Positive Control
Macrophages (in coronary atherosclerotic plaque), placenta tissue, Hofbauer cells (fetal macrophages), liver tissue
Negative Control
Lymphocytes, Monocytes
Preparation and Storage
Product should be stored at 4 degree C.
Under recommended storage conditions, product is stable for one year.
Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-MRC1 antibody
The monoclonal antibody 15-2 recognizes the mannose receptor (MR), also known as CD206, a member of the vertebrate C-type lectin family. The mannose receptor, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 175 kDa single-pass type I transmembrane receptor consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail.
The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR binds high-mannose structures on- a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator.
MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases with prolonged incubation time probably due to internalization of the MR-antibody-complex. The monoclonal antibody 15-2 prevents binding of glycoproteins including t-PA to MR.
Detection of the MR with anti-MR monoclonal antibody 15-2 can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.
The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR binds high-mannose structures on- a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator.
MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases with prolonged incubation time probably due to internalization of the MR-antibody-complex. The monoclonal antibody 15-2 prevents binding of glycoproteins including t-PA to MR.
Detection of the MR with anti-MR monoclonal antibody 15-2 can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.
Product Categories/Family for anti-MRC1 antibody
References
1. Barret-Berghoeff, M et al; Monoclonal antibodies against theHuman mannose receptor that inhibit the binding of tissue-type plasminogen activator. Thromb Haemostas 1997, 77: 718
2. Noorman, F et al; Monoclonal antibodies against theHuman mannose receptor as a specific marker in flow cytometry and immunohistochemistry for macrophages. J Leukocyte Biol 1997, 61: 63
3. Noorman, F et al; Cluster mannosides can inhibit mannose receptor-mediated tissue-type plasminogen activator degradation by both rat andHuman cells. Hepatology 1997, 26: 1303
4. Tailleux, L et al; DC-SIGN is the major mycobacterium tuberculosis receptor onHuman dendritic cells. J Exp Med 2003, 197: 121
5. Pressice, P et al; Keyhole limpet hemocyanin induces the activation and maturation ofHuman dendritic cells through the involvement of mannose receptor. Mol Immunol 2008, 45: 1136
6. Tahara, N et al; 2-deoxy-2-[(18)F]fluoro-d-mannose positron emission tomography imaging in atherosclerosis. Nat Med 2014, 20: 215
2. Noorman, F et al; Monoclonal antibodies against theHuman mannose receptor as a specific marker in flow cytometry and immunohistochemistry for macrophages. J Leukocyte Biol 1997, 61: 63
3. Noorman, F et al; Cluster mannosides can inhibit mannose receptor-mediated tissue-type plasminogen activator degradation by both rat andHuman cells. Hepatology 1997, 26: 1303
4. Tailleux, L et al; DC-SIGN is the major mycobacterium tuberculosis receptor onHuman dendritic cells. J Exp Med 2003, 197: 121
5. Pressice, P et al; Keyhole limpet hemocyanin induces the activation and maturation ofHuman dendritic cells through the involvement of mannose receptor. Mol Immunol 2008, 45: 1136
6. Tahara, N et al; 2-deoxy-2-[(18)F]fluoro-d-mannose positron emission tomography imaging in atherosclerosis. Nat Med 2014, 20: 215
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
macrophage mannose receptor 1
NCBI Official Synonym Full Names
mannose receptor C-type 1
NCBI Official Symbol
MRC1
NCBI Official Synonym Symbols
MMR; hMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1
NCBI Protein Information
macrophage mannose receptor 1
UniProt Protein Name
Macrophage mannose receptor 1
Protein Family
UniProt Gene Name
MRC1
UniProt Synonym Gene Names
CLEC13D; CLEC13DL; MRC1L1; MMR
UniProt Entry Name
MRC1_HUMAN
NCBI Description
The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.[provided by RefSeq, Sep 2015]
Uniprot Description
MRC1: Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.; Motility/polarity/chemotaxis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 10p12.33
Cellular Component: cell surface; integral to plasma membrane; plasma membrane; endosome membrane
Molecular Function: mannose binding; protein binding; transmembrane receptor activity; receptor activity
Biological Process: receptor-mediated endocytosis; signal transduction
Research Articles on MRC1
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Product Notes
The MRC1 mrc1 (Catalog #AAA584868) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mannose receptor, Human, mAb 15-2, biotinylated reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mannose receptor can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Frozen, Functional Studies (FS), Western Blot (WB). The typical starting working dilution is 1:50. IHC-F: Tissue sections were fixed in acetone/chloroform. Sections were preincubated with normal rabbit serum (Ref.3). FC: Antibody 15-2 stains the extracellular domain of MR. W: A non-reduced sample treatment and 6% SDS-Page was used. The band size is 175 kDa (Ref.1). FS: Antibody 15-2 functions as an inhibitor of binding. The antibody was functionally tested by preincubation of immobilized receptor with antibody. This prevented binding of t-PA to immobilized receptor. (Ref.1). Researchers should empirically determine the suitability of the MRC1 mrc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mannose receptor, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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