Rabbit Mannose Receptor/MRC1 Polyclonal Antibody | anti-MRC1 antibody

Anti-Mannose Receptor/MRC1 Antibody

Cat. Num. AAA1753033

• Gene Names: MRC1; MMR; hMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: Mannose Receptor/MRC1; Polyclonal Antibody; Anti-Mannose Receptor/MRC1 Antibody; Macrophage mannose receptor 1; MMR; C-type lectin domain family 13 member D; C-type lectin domain family 13 member D-like; Human mannose receptor; hMR; Macrophage mannose receptor 1-like protein 1; CD206; MRC1; CLEC13D; CLEC13DL; MRC1L1; anti-MRC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 1456

• Applicable Applications for anti-MRC1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml (Human, Mouse); IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse, Rat); FC/FACS: 1-3ug/1x106 cells (Human, Mouse); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.
• Immunogen: E Coli-derived human Mannose Receptor/MRC1 recombinant protein (Position: D21-A1140).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Flow Cytometry (FC/FACS)

Flow Cytometry (FC/FACS)

Flow Cytometry (FC/FACS)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Western Blot (WB)

(Figure 1. Western blot analysis of MRC1 using anti-MRC1 antibody.)

Related Product Information for anti-MRC1 antibody

Description: Rabbit IgG polyclonal antibody for Mannose Receptor/MRC1 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human; Mouse; Rat.

Background: The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages, immature dendritic cells and liver sinusoidal endothelial cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is mapped to 10p12.33. The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.

References

1. Eichbaum, Q., Clerc, P., Bruns, G., McKeon, F., Ezekowitz, R. A. B. Assignment of the human macrophage mannose receptor gene (MRC1) to 10p13 by in situ hybridization and PCR-based somatic cell hybrid mapping. Genomics 22: 656-658, 1994.
2. Harris, N., Peters, L. L., Eicher, E. M., Rits, M., Raspberry, D., Eichbaum, Q. G., Super, M., Ezekowitz, R. A. B. The exon-intron structure and chromosomal localization of the mouse macrophage mannose receptor gene, Mrc1: identification of a ricin-like domain at the N-terminus of the receptor. Biochem. Biophys. Res. Commun. 198: 682-692, 1994.
3. Kim, S. J., Ruiz, N., Bezouska, K., Drickamer, K. Organization of the gene encoding the human macrophage mannose receptor (MRC1). Genomics 14: 721-727, 1992.

NCBI and Uniprot Product Information

• NCBI GI #: 4505245
• NCBI GeneID: 4360
• NCBI Accession #: NP_002429.1
• NCBI GenBank Nucleotide #: NP_002429.1
• UniProt Accession #: P22897
• NCBI Official Full Name: macrophage mannose receptor 1
• NCBI Official Synonym Full Names: mannose receptor C-type 1
• NCBI Official Symbol: MRC1
• NCBI Official Synonym Symbols: MMR; hMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1
• NCBI Protein Information: macrophage mannose receptor 1
• UniProt Protein Name: Macrophage mannose receptor 1
• Protein Family: Macrophage mannose receptor
• UniProt Gene Name: MRC1
• UniProt Synonym Gene Names: CLEC13D; CLEC13DL; MRC1L1; MMR
• UniProt Entry Name: MRC1_HUMAN

NCBI Description

The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.[provided by RefSeq, Sep 2015]

Uniprot Description

MRC1: Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10p12.33

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; endosome membrane

Molecular Function: mannose binding; protein binding; transmembrane receptor activity; receptor activity

Biological Process: receptor-mediated endocytosis; signal transduction