Mouse anti-Human Azurocidin Monoclonal Antibody | anti-AZU1 antibody

Azurocidin, Human, mAb z6721

Cat. Num. AAA584765

• Gene Names: AZU1; AZU; HBP; NAZC; hHBP; AZAMP; CAP37; HUMAZUR
• Reactivity: Human
• Applications: Immuno Assay (IA)
• Synonyms: Azurocidin; Monoclonal Antibody; Human; mAb z6721; Cationic antimicrobial peptide CAP37; Heparin-binding protein; HBP; anti-AZU1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: z6721
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 251

• Applicable Applications for anti-AZU1 antibody: Immuno Assay (IA)
• Application Notes: IA: Antibody z6721 can be used both as detection and capture antibody.; The typical starting working dilution is 1:50.
• Preparation and Storage: Store at 4 degree C. Under recommended storage conditions, product is stable for at least one year.

Related Product Information for anti-AZU1 antibody

The monoclonal antibody z6721 recognizes azurocidin (also called heparin-binding protein (HBP) or cationic antimicrobial protein of 37 Kda (CAP37). Azurocidin is an inactive homolog of serine proteinases residing in granulocytes. Initially it was thought to have only activity against gram-negative bacteria. Later it was also found to act against gram-positive bacteria and C.albicans. Azurocidin is considered a family member of polymorphonuclear leukocytes- derived antimicrobial proteins like defensins, LL-37 and lysozyme. Azurocidin has been recognized as a player in the activation and modulation of the immune response and may act to alarm the immune system. The cationic domain at one side of the protein is essential for its antimicrobial activity. It is stored in azurophil granules as well as secretory granules and as a result partly released at early stage of extravasation. Azurocidin is involved in chemotaxis and activation of monocytes, cytokine release and phagocytosis leading to more efficient bacterial clearance. The primary targeted environment of azurocidin are cells in the bloodstream, that is the endothelial lining, and the extravascular surroundings. The interaction of azurocidin with leucocytes is mediated via beta2-intergrins. High plasma levels of azurocidin might help with the identification of patients which are at risk in developing sepsis.

NCBI and Uniprot Product Information

• NCBI GI #: 179302
• NCBI GeneID: 566
• Molecular Weight: 26,886 Da
• NCBI Official Full Name: azurocidin
• NCBI Official Synonym Full Names: azurocidin 1
• NCBI Official Symbol: AZU1
• NCBI Official Synonym Symbols: AZU; HBP; NAZC; hHBP; AZAMP; CAP37; HUMAZUR
• NCBI Protein Information: azurocidin
• UniProt Protein Name: Azurocidin
• Protein Family: Azurocidin
• UniProt Gene Name: AZU1
• UniProt Synonym Gene Names: hHBP

NCBI Description

Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. This gene encodes a preproprotein that is proteolytically processed to generate a mature azurophil granule antibiotic protein, with monocyte chemotactic and antimicrobial activity. It is also an important multifunctional inflammatory mediator. This encoded protein is a member of the serine protease gene family but it is not a serine proteinase, because the active site serine and histidine residues are replaced. The genes encoding this protein, neutrophil elastase 2, and proteinase 3 are in a cluster located at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, Nov 2015]

Uniprot Description

AZU1: This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram- negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G. Belongs to the peptidase S1 family. Elastase subfamily.

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: azurophil granule; extracellular space; extrinsic to membrane

Molecular Function: heparan sulfate proteoglycan binding; heparin binding; peptidase activity; protein binding; serine-type endopeptidase activity

Biological Process: antimicrobial humoral response; defense response to Gram-negative bacterium; defense response to virus; glial cell migration; microglial cell activation; monocyte activation; positive regulation of cell adhesion; positive regulation of fractalkine biosynthetic process; positive regulation of interleukin-1 beta biosynthetic process; positive regulation of MHC class II biosynthetic process; positive regulation of phagocytosis; positive regulation of protein kinase activity; positive regulation of tumor necrosis factor biosynthetic process; protein kinase C activation; protein processing; proteolysis