Testing Data
Human Azurocidin ELISA Kit | AZU1 elisa kit
Human Azurocidin Quick ELISA Kit
O.D.|0.059|0.138|0.202|0.332|0.560|0.969|1.857|2.321
n|16|16|16|
Mean(pg/ml)|191|617|1152|
Standard deviation|10.12|38.87|81.79|
CV(%)|5.3%|6.3%|7.1%|
n|24|24|24
Mean(pg/ml)|185|604|1089
Standard deviation|9.99|39.86|95.83
CV(%)|5.4%|6.6%|8.8%
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 191|180|187|202|190|10.83|5.7%
Sample 2: 617|589|606|634|612|40.97|6.7%
Sample 3: 1152|1198|1201|1057|1152|48.38|4.2%
*number of samples for each test n=16.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
To measure Human AZU1, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human AZU1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human AZU1 in the sample.
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI Description
Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. The protein encoded by this gene is an azurophil granule antibiotic protein, with monocyte chemotactic and antibacterial activity. It is also an important multifunctional inflammatory mediator. This encoded protein is a member of the serine protease gene family but it is not a serine proteinase, because the active site serine and histidine residues are replaced. The genes encoding this protein, neutrophil elastase 2, and proteinase 3 are in a cluster located at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, Jul 2008]
Uniprot Description
AZU1: This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram- negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G. Belongs to the peptidase S1 family. Elastase subfamily.
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: azurophil granule; extracellular region
Molecular Function: heparin binding; toxin binding; serine-type endopeptidase activity
Biological Process: positive regulation of tumor necrosis factor biosynthetic process; positive regulation of cell adhesion; microglial cell activation; glial cell migration; proteolysis; defense response to Gram-negative bacterium; macrophage chemotaxis; induction of positive chemotaxis; cellular extravasation; monocyte activation; positive regulation of MHC class II biosynthetic process; positive regulation of phagocytosis; protein kinase C activation; positive regulation of fractalkine biosynthetic process; positive regulation of interleukin-1 beta biosynthetic process; immune response; protein processing; regulation of vascular permeability; inflammatory response; negative regulation of apoptosis
Research Articles on AZU1
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Product Notes
The Human AZU1 azu1 (Catalog #AAA1753948) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1753948 ELISA Kit recognizes Human AZU1. It is sometimes possible for the material contained within the vial of "Azurocidin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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