Rat anti-Mouse CD96 Monoclonal Antibody | anti-CD96 antibody

CD96, Mouse, mAb 6A6

Cat. Num. AAA584711

• Gene Names: CD96; TACTILE
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Synonyms: CD96; Monoclonal Antibody; Mouse; mAb 6A6; TACTILE; anti-CD96 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 6A6
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 585

• Applicable Applications for anti-CD96 antibody: Flow Cytometry (FC/FACS), Functional Studies (FS), Immuno Assay (IA), Western Blot (WB)
• Application Notes: The typical starting working dilution is 1:50. For functional studies dilutions have to be made according to the amounts of CD96 to be inactivated.
• Preparation and Storage: Store at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-CD96 antibody

The monoclonal antibody 6A6 recognizes mouse CD96. CD96 or T cell-Activated Increased Late Expression (TACTILE) is an ~67 kD immunoglobulin-like protein expressed on natural killer (NK) and T cells. CD96 belongs to the Ig-super family, consisting of three Ig-like domains and an extended serine/threonine/praline-rich stalk in the ectodomain. CD96 is upregulated upon T cell activation and is involved in engagement of diseased cells and cell movement towards areas of inflammation. Mouse CD96 binds to CD155 and nectin-1. CD96/CD155-interaction is preserved between man and mouse although both receptors are only moderately conserved in amino acid sequence. The monoclonal antibody 6A6 completely inhibits binding of CD96 to both recombinant mouse CD155 and mouse nectin-1. Also, T cell adhesion to insect cells expressing mCD155 is blocked by this monoclonal antibody, however, depending on the T cell subtype. The monoclonal antibody 6A6 was raised after immunization of rats with purified recombinant mCD96-hIgG1.

References

1. Seth, S et al; The murine pan T cell marker CD96 is an adhesion receptor for CD155 and nectin-1. Biochem Biophys Res Commun 2007, 364: 959 2. Maier, M et al; The adhesion receptor CD155 determines the magnitude of humoral immune responses against orally ingested antigens. Eur J Immunol 2007, 37: 2214

NCBI and Uniprot Product Information

• NCBI GI #: 119600104
• NCBI GeneID: 10225
• Molecular Weight: 63,888 Da
• NCBI Official Full Name: CD96 antigen, isoform CRA_c
• NCBI Official Synonym Full Names: CD96 molecule
• NCBI Official Symbol: CD96
• NCBI Official Synonym Symbols: TACTILE
• NCBI Protein Information: T-cell surface protein tactile
• UniProt Protein Name: T-cell surface protein tactile
• Protein Family: T-cell surface protein tactile
• UniProt Gene Name: CD96

NCBI Description

The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein. The protein may play a role in the adhesive interactions of activated T and NK cells during the late phase of the immune response. It may also function in antigen presentation. Alternative splicing generates multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2016]

Uniprot Description

CD96: May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation. Defects in CD96 are a cause of C syndrome (CSYN); also called Opitz trigonocephaly syndrome. This syndrome is characterized by trigonocephaly and associated anomalies, such as unusual facies, wide alveolar ridges, multiple buccal frenula, limb defects, visceral anomalies, redundant skin, psychomotor retardation and hypotonia. A chromosomal aberration involving CD96 has been found in a patient with C syndrome. Translocation t(3;18)(q13.13;q12.1). CD96 gene was located at the 3q13.13 breakpoint. Precise structural analysis around the breakpoint showed that the gene was disrupted by the translocation in exon 5, probably leading to premature termination or loss of expression of CD96 protein. No gene was detected at the chromosome 18 breakpoint. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13.13-q13.2

Cellular Component: adherens junction; cytoplasm; integral to plasma membrane; plasma membrane

Biological Process: cell adhesion; cell-matrix adhesion; immune response; regulation of immune response

Disease: C Syndrome