Mouse anti-Human PDZ and LIM Domain 1 Monoclonal Antibody | anti-PDLIM1 antibody

PDZ and LIM Domain 1

Cat. Num. AAA4381378

• Gene Names: PDLIM1; CLIM1; CLP36; CLP-36; hCLIM1; HEL-S-112
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: PDZ and LIM Domain 1; Monoclonal Antibody; C terminal LIM domain protein 1; carboxy terminal LIM domain protein 1; CLIM1; CLP36; Elfin; Enigma antibody; Enigma homolog antibody; hCLIM1; PDLIM1; PDZ and LIM domain 1; anti-PDLIM1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: CPTC-PDLIM1-1
• Specificity: CLP36, also known as PDLIM1, CLIM1 or Elfin, is a 329 amino acid cytoplasmic protein that associates with actin stress fibers at the cytoskeleton. Expressed at high levels in skeletal muscle and heart and at lower levels in colon, small intestine, spleen, lung, placenta, kidney, liver, thymus and pancreas, CLP36 functions as a cytoskeletal protein that is thought to act as an adaptor, bringing target proteins to the cytoskeleton. Specifically, CLP36 interacts with Clik1 (-actinin-1, thereby facilitating the association of Clik1 with actin stress fibers. CLP36 contains one PDZ domain and one LIM zinc-binding domain through which it conveys its protein-protein binding capabilities. Human CLP36 shares 88% sequence similarity with its rat counterpart, suggesting a conserved function between species.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 329

• Applicable Applications for anti-PDLIM1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: WB: 0.5-1.0ug/ml; Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: HepG2 cells and extracts from Jurkat. Human heart, colon or spleen.
• Immunogen: Recombinant human full-length PDLIM1 protein
• Cellular Localization: Cytoplasm.
• Hu-chromosome Location: 10q23.33
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with PDZ and LIM Domain 1 Mouse Monoclonal Antibody (PDLIM-1).)

SDS-Page

(SDS-PAGE Analysis Purified PDZ and LIM Domain 1 Mouse Monoclonal Antibody (PDLIM-1). Confirmation of Purity and Integrity of Antibody)

References

Bauer, K., et al. 2000. Blood 96: 4236-4245

NCBI and Uniprot Product Information

• NCBI GI #: 13994151
• NCBI GeneID: 9124
• NCBI Accession #: NP_066272.1
• NCBI GenBank Nucleotide #: NP_066272.1
• UniProt Accession #: O00151
• Molecular Weight: 36kDa
• NCBI Official Full Name: PDZ and LIM domain protein 1
• NCBI Official Synonym Full Names: PDZ and LIM domain 1
• NCBI Official Symbol: PDLIM1
• NCBI Official Synonym Symbols: CLIM1; CLP36; CLP-36; hCLIM1; HEL-S-112
• NCBI Protein Information: PDZ and LIM domain protein 1
• UniProt Protein Name: PDZ and LIM domain protein 1
• Protein Family: PDZ and LIM domain protein
• UniProt Gene Name: PDLIM1
• UniProt Synonym Gene Names: CLIM1; CLP36
• UniProt Entry Name: PDLI1_HUMAN

NCBI Description

This gene encodes a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17. [provided by RefSeq, Oct 2012]

Uniprot Description

CLIM1: a cytoskeletal adaptor protein that bring other proteins to the cytoskeleton. Interacts with alpha-actinins 1, 2 and 4. Recruits the Clik1 kinase to actin stress fibers in nonmuscle cells. Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 10q23.1

Cellular Component: transcription factor complex; cytoskeleton; focal adhesion; cytoplasm

Molecular Function: zinc ion binding; transcription coactivator activity

Biological Process: regulation of transcription, DNA-dependent; response to hypoxia; response to oxidative stress