Mouse SAA-1 Monoclonal Antibody | anti-SAA-1 antibody

SAA-1, Human, mAb Reu86.5

Cat. Num. AAA584371

• Gene Names: Saa1; Saa2; Saa-1
• Reactivity: Reactivity: Human. Cross reactivity: Mouse
• Applications: Western Blot
• Synonyms: SAA-1; Monoclonal Antibody; Human; mAb Reu86.5; anti-SAA-1 antibody

• Host: Mouse
• Reactivity: Reactivity: Human. Cross reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 122

• Applicable Applications for anti-SAA-1 antibody: F, Immunoasssay (IA), P, Western Blot
• Application Notes: Application Use: For western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-SAA-1 antibody

Product Description: The serum amyloid A (SAA) family comprises a number of differentially expressed apolipoproteins, acute-phase SAA1 and SAA2, the former being the major component in plasma, and constitutive SAAs (C-SAAs). Although the liver is the primary site of synthesis of both SAA types extrahepatic production has been reported. The in vivo concentrations increase by as much as 1000-fold during inflammation. Several studies have stressed its importance in the identification and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations; SAA rises earlier and more sharply than CRP.
Recently, a broader view of SAA expression and function has been emerging. Expression studies show production of SAA proteins in histologically normal, atherosclerotic, Alzheimer, inflammatory, and tumor tissues. SAA has been found to have binding sites for high density lipoproteins, calcium, laminin, and heparin/heparan-sulfate. Also adhesion motifs were identified and new functions, affecting cell adhesion, migration, proliferation and aggregation discovered. These findings emphasize the importance of SAA in various physiological and pathological processes, including inflammation, atherosclerosis, thrombosis, AA-amyloidosis, rheumatoid arthritis, and neoplasia. SAA has also a number of immunomodulatory roles, it can induce chemotaxis and adhesion molecule expression, has cytokine-like properties and can promote the upregulation of metalloproteinases. It enhances the binding of high-density lipoprotein to macrophages and thus helps in the delivery of lipids to sites of injury for use in tissue repair. It is thus thought to be an integral part of the disease processes. In addition, recent experiments suggest that SAA may play a "housekeeping" role in normal human tissues.
Elevated levels of SAA over time predispose to secondary amyloidosis, extracellular accumulation of amyloid fibrils, derived from a circulating precursor, in various tissue and organs. The most common form of amyloidosis occurs secondary to chronic inflammatory disease, particularly rheumatoid arthritis.
The antibody is raised against human SAA and Helix Pomatia Haemocyanine. It reacts with the SAA-1 type.

References

1. Hazenberg, B et al; Immunohistochemical detection of Amyloid AA in formaline-fixed paraffin-embedded rectal biopsies with the monoclonal antibody anti-human SAA antibody Reu.86.2. Amyloid and Amyloidosis 1990, VIth International Symposium on Amyloidosis: 809 2. Hazenberg, B et al; Monoclonal antibody based ELISA for human SAA. Amyloid and Amyloidosis, eds. LB Natvig et al, Kluwer Acad Publ 1990: 898
3. Wilkins, J et al; Rapid automated enzyme immunoassays of Serum Amyloid A. Clin Chem 1994, 40: 1284
4. Hazenberg, B et al; A quantitative method for detecting deposits of amyloid A protein in aspirated fat tissue of patients with arthritis. Ann Rheum Dis 1999, 58: 96

NCBI and Uniprot Product Information

• NCBI GI #: 6677843
• NCBI GeneID: 20208
• NCBI Accession #: NP_033143.1
• NCBI GenBank Nucleotide #: NM_009117.3
• Molecular Weight: 13,770 Da
• NCBI Official Full Name: serum amyloid A-1 protein
• NCBI Official Synonym Full Names: serum amyloid A 1
• NCBI Official Symbol: Saa1
• NCBI Official Synonym Symbols: Saa2; Saa-1
• NCBI Protein Information: serum amyloid A-1 protein; serum amyloid A 2
• UniProt Protein Name: Serum amyloid A-1 protein
• UniProt Gene Name: Saa1
• UniProt Entry Name: SAA1_MOUSE

Uniprot Description

SAA1: Major acute phase reactant. Apolipoprotein of the HDL complex. Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA1 protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Elevated serum SAA1 protein levels may be associated with lung cancer. Belongs to the SAA family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; chemoattractant activity

Biological Process: cholesterol metabolic process; acute-phase response