Mouse SAA ELISA Kit | SAA1 elisa kit

Mouse SAA ELISA Kit

Cat. Num. AAA824614

• Gene Names: Saa1; Saa2; Saa-1
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: SAA; Mouse SAA ELISA Kit; Serum amyloid A-1 protein; SAA1 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse SAA ELISA Kitallows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse SAA proteins within the range of 1,560 pg/ml-100,000 pg/ml
• Sequence Length: 122

• Applicable Applications for SAA1 elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma, Urine
• Sensitivity: <800 pg/mL
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SAA1 elisa kit

Principle of the Assay: The MBSMouse SAA ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse SAAin Cell Culture Supernatants, Serum, Plasma, Urine. This assay employs an antibody specific for Mouse SAAcoated on a 96-well plate. Standards and samples are pipetted into the wells and SAApresent in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse SAAantibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SAAbound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Serum amyloid A protein (SAA), also known as SAA1, is a protein that inhumans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.

NCBI and Uniprot Product Information

• NCBI GI #: 6677843
• NCBI GeneID: 20208
• NCBI Accession #: NP_033143.1
• NCBI GenBank Nucleotide #: NM_009117.3
• UniProt Accession #: P05366
• Molecular Weight: 13,770 Da
• NCBI Official Full Name: serum amyloid A-1 protein
• NCBI Official Synonym Full Names: serum amyloid A 1
• NCBI Official Symbol: Saa1
• NCBI Official Synonym Symbols: Saa2; Saa-1
• NCBI Protein Information: serum amyloid A-1 protein; serum amyloid A 2
• UniProt Protein Name: Serum amyloid A-1 protein
• Protein Family: Serum amyloid A-1 protein
• UniProt Gene Name: Saa1
• UniProt Entry Name: SAA1_MOUSE

Uniprot Description

SAA1: Major acute phase reactant. Apolipoprotein of the HDL complex. Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA1 protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Elevated serum SAA1 protein levels may be associated with lung cancer. Belongs to the SAA family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; chemoattractant activity

Biological Process: cholesterol metabolic process; acute-phase response