BrdU IHC Kit
BrdU Immunohistochemistry Kit
Synonyms
BrdU; BrdU Immunohistochemistry Kit; BrdU ihc kit
Application Notes
Immunohistochemistry on formalin fixed, paraffin embedded, as well as frozen tissue sections. Furthermore this kit can be used for the immunocytochemical staining of cultured cells on glass slides or cell culture plates. Optimal concentration of the primary antibody should be evaluated by serial dilutions.
Note
The BrdU Cell Immunohistochemistry Kit contains all ingredients (antibodies, buffers and detection reagents) needed for the immunohistochemical detection and quantification of the proliferative activity of cells and tissues. The kit contains 5 control slides with BrdU prelabeled MCF-7 cells (one of which is prestained) as well as an instruction manual describing the relevant protocols for BrdU immunostaining.
Preparation and Storage
Upon receipt, store entire kit at -20oC. Once the kit is thawed, you may keep it at 4 degree C for 5 days. For long-term storage, it is recommended you aliquot and freeze the components at –20 degree C, particularly the Streptavidin-HRP Conjugate, the Prediluted Biotinylated Sheep anti-BrdU Detector Antibody and the 4x Trypsin Concentrate.
Related Product Information for BrdU ihc kit
The BrdU Immunohistochemistry Kit is a non-isotopic enzyme immunoassay for the detection and quantification of DNA synthesis and cell proliferation. Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S-phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment. A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators. In these methods bromodeoxyuridine (BrdU), a thymidine analog, replaces [3H] thymidine. BrdU is incorporated into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells which are actively synthesizing DNA. Exalpha Biologicals BrdU Immunohistochemistry Kit involves incorporation of BrdU Reagent into cells, either in tissues after injection of BrdU in experimental animals or in cells cultured in microtiter plates or on glass slides. The resultant assay is, rapid, easy to perform and applicable to high sample throughput. In addition to evaluation of cell proliferation, information such as cell number, morphology and analysis of cellular antigens can be obtained from a single culture or tissue section.
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Product Notes
The BrdU (Catalog #AAA574011) is an IHC Kit and is intended for research purposes only. The product is available for immediate purchase. Immunohistochemistry on formalin fixed, paraffin embedded, as well as frozen tissue sections. Furthermore this kit can be used for the immunocytochemical staining of cultured cells on glass slides or cell culture plates. Optimal concentration of the primary antibody should be evaluated by serial dilutions. Researchers should empirically determine the suitability of the BrdU for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BrdU, IHC Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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