Rabbit cortisol binding globulin Polyclonal Antibody | anti-SERPINA6 antibody

Rabbit anti Human cortisol binding globulin

Cat. Num. AAA573049

• Gene Names: SERPINA6; CBG
• Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail.
• Applications: Immunoprecipitation
• Synonyms: cortisol binding globulin; Polyclonal Antibody; Rabbit anti Human cortisol binding globulin; anti-SERPINA6 antibody

• Host: Rabbit
• Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail.
• Clonality: Polyclonal
• Specificity: In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. The antiserum does not react with any other protein component of human serum or plasma.
• Form/Format: Delipidated, heat inactivated, lyophilized, stable whole serum.

• Applicable Applications for anti-SERPINA6 antibody: Immunoprecipitation (IP)
• Protein concentration: Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added.
• Preservative: No preservative added.
• Immunogen: Cortisol binding globulin is an alpha-1 glycoprotein with a molecular weight of 55,700. It is bonding and transporting cortisol in plasma. The mean concentration in adult serum is about 50 ug/ml. Elevated levels are observed in pregnancy. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
• Reactivity Note: Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail.
• Antibody titre: Precipitin titre not less than 1:16 when tested against normal human serum in agar-block immunodiffusion titration.
• Intended use: In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of cortisol binding globulin or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques as ELISA and Western blotting if proper controls are included.
• Directions for use: In immunoelectrophoresis use 2 III human plasma or equivalent against 120 III antiserum. In double radial immunodiffusion use a rosette arrangement with 10 III antiserum in 3 mm diameter center well and 2 III plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion use 1 percent antiserum in the gel.
• Packing: Vial with 1 mllyophilized antiserum.
• Caution: This antiserum should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro research purposes only.
• Preparation and Storage: Lyophilized at +4 degree C = at least 10 years; Reconstituted at or below -20 degree C = 3-5 years; Reconstituted at +4 degree C = 7 days; ; Handling: ; The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum.; Diluted antiserum should be stored at +4 degree C, not refrozen, and preferably used the same day.

Related Product Information for anti-SERPINA6 antibody

Precipitating polyclonal rabbit antiserum to human cortisol binding globulin (transcortin)

Product Categories/Family for anti-SERPINA6 antibody

Antisera

NCBI and Uniprot Product Information

• NCBI GI #: 73858564
• NCBI GeneID: 866
• NCBI Accession #: NP_001747.2
• NCBI GenBank Nucleotide #: NM_001756.3
• UniProt Accession #: P08185; Q7Z2Q9; A8K456
• Molecular Weight: 45,141 Da
• NCBI Official Full Name: corticosteroid-binding globulin
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6
• NCBI Official Symbol: SERPINA6
• NCBI Official Synonym Symbols: CBG
• NCBI Protein Information: corticosteroid-binding globulin; serpin A6; transcortin; corticosteroid binding globulin; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6
• UniProt Protein Name: Corticosteroid-binding globulin
• Protein Family: Corticosteroid-binding globulin
• UniProt Gene Name: SERPINA6
• UniProt Synonym Gene Names: CBG; CBG
• UniProt Entry Name: CBG_HUMAN

NCBI Description

This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events. [provided by RefSeq, Jul 2008]

Uniprot Description

SERPINA6: Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species. Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency). CBG deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo/hypertension and muscle fatigue. Belongs to the serpin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity; steroid binding

Biological Process: glucocorticoid metabolic process; transport

Disease: Corticosteroid-binding Globulin Deficiency