Mononucleosomes H3.2 Recombinant Protein | H3.2 recombinant protein

Recombinant Mononucleosomes H3.2

Cat. Num. AAA389389

• Synonyms: Mononucleosomes H3.2; Recombinant Mononucleosomes H3.2; H3.2 recombinant protein

• Host: E Coli
• Form/Format: Recombinant Mononucleosomes H3.2 is supplied in 10mM Tris-HCl pH8.0, 1mM EDTA, 2mM DTT, and 20% glycerol.

• Protein Species: Human
• Protein Details: Recombinant Mononucleosomes H3.2 consist of a 167 bp of 601 DNA and two molecules each of histones H2A that includes amino acids 1-130 (end)(accession number NM_003512), H2B that includes amino acids 1-126 (end)(accession number NM_003518), H3.2 that includes amino acids 1-136 (end)(accession number NM_021059.3), and H4 that includes amino acids 1-103 (end)(accession number NM_003548). All of these histones were expressed in E Coli cells.
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data this product is shown.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant Mononucleosomes H3.2 12.5% SDS-PAGE with Coomassie staining MW: 108 kDa Purity: >95%)

Testing Data

(Recombinant Mononucleosomes H3.2 DNA gel Recombinant Mononucleosomes H3.2 were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: Free 601 DNA which was used for assembly of nucleosome. Lane 3: Intact mononucleosomes H3.2. Intact mononucleosomes H3.2 migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrapped histone octamers to form nucleosomes.)

Related Product Information for H3.2 recombinant protein

Background: In vivo, histones are wrapped around by DNA in chromatin. Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are signifcantly more active, as well as specifc, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery. Nuclear core histone H3 exist as several variants: H3.1, H3.2, H3.3, CENP-A and H3t. These variants have been suggested to have specific functions in the regulation of gene expression and genome stability. In mammals, histone H3 non-centromeric variants, H3.1, H3.2 and H3.3, differ in their chromatin deposition patterns and PTMs despite having a high degree of sequence similarity. H3.1 and H3.2, which are enriched in repressive chromatin marks, are predominantly expressed during S-Phase and deposited in DNA synthesis-coupled fashion during DNA replication and repair. In contrast, H3.3 is enriched in active chromatin marks and is expressed throughout the cell cycle. Canonical histone H3.2 is expressed during S phase and differs by only one amino acid residue from th variant histone H3.1, and four amino acid residues from histone H3.3. According to some researchers, H3.1 is preferentially acetylated and dimethylated on lysines 14 and 9, respectively, while H3.2 is preferentially subjected to trimethylation on lysine 27.

Product Categories/Family for H3.2 recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GenBank Nucleotide #: NM_003512
• Molecular Weight: Molecular weight of the histone octamer is 108kDa