Mouse anti-Human Lysozyme (FITC) and Lactoferrin (PE) Antibody

COMBI IC Reagent: Mouse anti Lysozyme (FITC) and Mouse anti Lactoferrin (PE)

Cat. Num. AAA570350

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: Lysozyme (FITC) and Lactoferrin (PE); COMBI IC Reagent: Mouse anti Lysozyme (FITC) and Mouse anti Lactoferrin (PE); anti-Lysozyme (FITC) and Lactoferrin (PE) antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1
• Clone Number: LZ-2; 4C5
• Specificity: The anti-Lysozyme Antibody (clone LZ-2) reacts with intracellular human lysozyme/muramidase expressed by virtually all; myelomonocytic cells, macrophages and their precursors. The LF mAb (clone 4C5) recognizes lactoferrin stored within; secondary granules of postmitotic granulocyte-commited cells.; ; In this COMBI-IC Reagent antibody LZ-2 is conjugated to FITC, antibody 4C5 is conjugated to Phycoeythrin (PE).; ; The sensitivity y of LZ/LF mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAbdilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb-concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilutionexpected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ul of leukocytes containing 10^7 cells/ml are stained with 20ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.
• Form/Format: PBS pH 7.2, 1% BSA, 0.05% NaN3

• Applicable Applications for anti-Lysozyme (FITC) and Lactoferrin (PE) antibody: Flow Cytometry (FC/FACS)
• Application Notes: Permeabilization and Staining Procedure; - In combination with our Permeabilization Kit FIX&PERM intracellular Lysozyme and Lactoferrin can be easily stained in cell suspensions.; - For each sample to be analyzed add 50 ul of whole blood, bone marrow or mononuclear cell suspension in a 5ml tube; - Add 100 ul of Reagent A (Fixation Medium, stored and used at room temperature); - Incubate for 15 minutes at room temperature; - Add 5ml phosphate buffered saline and centrifuge cells for 5 minutes at 300 g; - Remove supernatant and add to cell pellet 100 ul Reagent B (Permeabilization Medium) and 20 ul of the LZ/LF COMBI-IC monoclonal antibody conjugate; - Vortex at low speed for 1-2 seconds; - Incubate for 15 minutes at room temperature; - Wash cells with phosphate buffered saline as described above; - Remove supernatant and resuspend cells in sheath fluid for immediate analysis or resuspend cells in 0.5 ml 1.0 %; formaldehyde and store them at 2-8 degree C in the dark. Analyze fixed cells within 24 hours.
• CE Mark: CE
• Conjugation: FITC and PE
• Preparation and Storage: Monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8 degree C (DO NOT FREEZE!) and protected from prolonged exposure to light. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.

Related Product Information for anti-Lysozyme (FITC) and Lactoferrin (PE) antibody

Lysozyme (LZ) is a cationic antimicrobial peptide of 14 kDa. Lysozyme is stored in primary but predominantly in specific (secondary) granules of neutrophils. It cleaves peptidoglycan constituents of the bacterial cell wall and can bind LPS. The epitope recognized by antibody LZ-2 is expressed by virtually all myeloid cells including normal and malignant granulocytes and monocytes. In normal myelopoiesis LZ can first be detected at the myeloblast stage where it appears somewhat later than MPO expression. Lactoferrin (LF) is an iron-binding protein with bactericidal and bacteriostatic activity, which is stored within the secondary granules of granulocytes. LF expression is restricted to the post-mitotic maturation compartment of the granulocytic lineage, starting from the myelocyte stage. Normal and malignant myeloblasts are LF negative. The combined staining for Lysozyme and Lactoferrin allows the distinction between mature and immature myelomonocytic cells.

The LZ/LF COMBI-IC reagent permits the identification and enumeration of myeloid cells in normal and malignant human blood and bone marrow samples using flow cytometry. Results must be put within the context of other tests as well as the clinical history of the patient by a certified professional before final interpretation. Analysis performed with this antibody should be paralleled by positive and negative controls.

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