Rabbit anti-Human, Mouse IFIH1 / MDA5 Polyclonal Antibody | anti-IFIH1 antibody

Rabbit Polyclonal to Human IFIH1 / MDA5

Cat. Num. AAA241888

• Gene Names: IFIH1; AGS7; Hlcd; MDA5; MDA-5; RLR-2; IDDM19
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: IFIH1 / MDA5; Polyclonal Antibody; Rabbit Polyclonal to Human IFIH1 / MDA5; Anti-IFIH1 / MDA5 Antibody (C-Terminus) IHC-plus; IFIH1; Helicase with 2 CARD domains; MDA-5; MDA5; Helicard; IDDM19; RLR-2; RH116; CADM-140 autoantigen; Hlcd; RIG-I-like receptor 2; Human IFIH1; anti-IFIH1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: 16 amino acid peptide from near the carboxy terminus of human MDA5.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1025

• Applicable Applications for anti-IFIH1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB (4 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and a panel of mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After inc...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human IFIH1 / MDA5.
• Immunogen Type: Synthetic peptide
• Immunogen: IFIH1 / MDA5 antibody was raised against synthetic peptide from human IFIH1 / MDA5.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-MDA5 antibody IHC of mouse pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-MDA5 antibody IHC of mouse small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-MDA5 antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-MDA5 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.)

Related Product Information for anti-IFIH1 antibody

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Product Categories/Family for anti-IFIH1 antibody

Family: DEAD box protein

NCBI and Uniprot Product Information

• NCBI GI #: 27886568
• NCBI GeneID: 64135
• NCBI Accession #: NP_071451.2
• NCBI GenBank Nucleotide #: NM_022168.3
• UniProt Accession #: Q9BYX4; Q2NKL6; Q6DC96; Q86X56; Q96MX8; Q9H3G6
• Molecular Weight: 25,129 Da
• NCBI Official Full Name: interferon-induced helicase C domain-containing protein 1
• NCBI Official Synonym Full Names: interferon induced with helicase C domain 1
• NCBI Official Symbol: IFIH1
• NCBI Official Synonym Symbols: AGS7; Hlcd; MDA5; MDA-5; RLR-2; IDDM19
• NCBI Protein Information: interferon-induced helicase C domain-containing protein 1; helicard; CADM-140 autoantigen; RIG-I-like receptor 2; helicase with 2 CARD domains; RNA helicase-DEAD box protein 116; murabutide down-regulated protein; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; melanoma differentiation associated protein-5; melanoma differentiation-associated protein 5; clinically amyopathic dermatomyositis autoantigen 140 kDa
• UniProt Protein Name: Interferon-induced helicase C domain-containing protein 1
• Protein Family: Interferon-induced helicase C domain-containing protein
• UniProt Gene Name: IFIH1
• UniProt Synonym Gene Names: MDA5; RH116; CADM-140 autoantigen; Helicard; MDA-5; RLR-2
• UniProt Entry Name: IFIH1_HUMAN

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. Genetic variation in this gene is associated with diabetes mellitus insulin-dependent type 19. [provided by RefSeq, Jul 2012]

Uniprot Description

IFIH1: Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O- methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines. Monomer in the absence of ligands and homodimerizes in the presence of dsRNA ligands. Can assemble into helical or linear polymeric filaments on long dsRNA. Interacts with MAVS/IPS1. Interacts with V protein of Simian virus 5, Human parainfluenza virus 2, Mumps virus, Sendai virus and Hendra virus. Binding to paramyxoviruses V proteins prevents IFN-beta induction, and the further establishment of an antiviral state. Interacts with PCBP2. Interacts with NLRC5. Interacts with PIAS2-beta. Interacts with DDX60. By interferon (IFN) and TNF. Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung. Belongs to the helicase family. RLR subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Helicase; EC 3.6.4.13; Apoptosis

Chromosomal Location of Human Ortholog: 2q24

Cellular Component: cytosol; nucleus

Molecular Function: ribonucleoprotein binding; protein binding; single-stranded RNA binding; DNA binding; zinc ion binding; double-stranded RNA binding; helicase activity; ATP binding

Biological Process: regulation of apoptosis; protein sumoylation; detection of virus; viral reproduction; response to virus; positive regulation of interferon-beta production; innate immune response; negative regulation of interferon type I production; positive regulation of interferon-alpha production

Disease: Aicardi-goutieres Syndrome 7; Singleton-merten Syndrome 1