Testing Data
(Figure 1: Immunohistochemistry on paraffin section of human heart
)
Mouse anti-Human alphaT- Catenin Monoclonal Antibody | anti-CTNNA3 antibody
Mouse anti alphaT- Catenin
Gene Names
CTNNA3; VR22
Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
alphaT- Catenin; Monoclonal Antibody; Mouse anti alphaT- Catenin; anti-CTNNA3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
892_24D2S
Specificity
892_24D2S reacts with an epitope loCated between residues 164-177 of the Human alphaT-Catenin protein.
Form/Format
The vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-CTNNA3 antibody
Flow Cytometry (FC), Immunocytochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
892_24D2S is suitable for immunofluorescence (aceton-fixed cells), immunocytochemistry (methanol and paraformaldehyde-fixed cells, followed by permeabilisation in 0.2% Triton-X 100 for 2 min), immunoprecipitation and immunoblotting. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:50 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.
Source Note
892_24D2S is a Mouse monoclonal IgG2a antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a C57Bl/6 Mouse immunized with a synthetic peptide corresponding to amino acids 164-177 of the Human alphaT-Catenin protein including an additional N-terminal cysteine (CVANKSDLQKTYQKL) coupled to keyhole limpet hemocyanin.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Testing Data
(Figure 1: Immunohistochemistry on paraffin section of human heart )
Testing Data
(Figure 1: Immunohistochemistry on paraffin section of human heart
)
Related Product Information for anti-CTNNA3 antibody
Catenins are proteins that connect cadherin/beta-Catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, alphaT-Catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed Human alphaT-Catenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that alphaT-Catenin can functionally restore cell-cell adhesion in colon cancer cells lacking alpha-Catenins. This indiCates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other alpha-Catenins to which it shows an overall amino acid identity of 57%. In cells transfected with alphaT-Catenin cDNA, interaction with beta-Catenin was demonstRated by co-immunoprecipitation. The 892_24D2S antibody anti alphaT-Catenin does not show any cross reaction with alphaE-Catenin or alphaN-Catenin in western blot analysis.
Product Categories/Family for anti-CTNNA3 antibody
References
Janssens, B., Goossens, S., Staes, K., Gilbert, B., van Hengel, J., Colpaert, C., Bruyneel, E., Mareel, M., and van Roy, F. (2001). alphaT-Catenin: a novel tissue-specific beta-Catenin-binding protein mediating strong cell-cell adhesion, J. Cell. Sci. 114, 3177-88.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
99,809 Da
NCBI Official Full Name
alphaT-catenin
NCBI Official Synonym Full Names
catenin (cadherin-associated protein), alpha 3
NCBI Official Symbol
CTNNA3
NCBI Official Synonym Symbols
VR22
NCBI Protein Information
catenin alpha-3; alpha T-catenin; alpha-T-catenin; alpha-catenin-like protein; cadherin-associated protein
UniProt Protein Name
Catenin alpha-3
Protein Family
UniProt Gene Name
CTNNA3
UniProt Entry Name
CTNA3_HUMAN
NCBI Description
This gene encodes a protein that belongs to the vinculin/alpha-catenin family. The encoded protein plays a role in cell-cell adhesion in muscle cells. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia, familial 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
Uniprot Description
CTNNA3: May be involved in formation of stretch-resistant cell- cell adhesion complexes. Belongs to the vinculin/alpha-catenin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion
Chromosomal Location of Human Ortholog: 10q22.2
Cellular Component: cytoskeleton; lamellipodium; cytoplasm; fascia adherens
Molecular Function: actin filament binding; protein binding; cadherin binding
Biological Process: cell-cell adhesion
Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 13
Research Articles on CTNNA3
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Product Notes
The CTNNA3 ctnna3 (Catalog #AAA570076) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti alphaT- Catenin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's alphaT- Catenin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunocytochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). 892_24D2S is suitable for immunofluorescence (aceton-fixed cells), immunocytochemistry (methanol and paraformaldehyde-fixed cells, followed by permeabilisation in 0.2% Triton-X 100 for 2 min), immunoprecipitation and immunoblotting. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:50 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations. Researchers should empirically determine the suitability of the CTNNA3 ctnna3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alphaT- Catenin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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