Testing Data
(Image: TCF4 (C9B9) staining of HCT-116 cells (left) or SHSY-5Y cells (right) using TCF4 (C9B9) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor; 555 phalloidin (red).)
TCF4 (C9B9) Antibody | anti-TCF4 antibody
TCF4 (C9B9), Monoclonal Antibody, Rabbit
Testing Data
(Image: TCF4 (C9B9) staining of HCT-116 cells (left) or SHSY-5Y cells (right) using TCF4 (C9B9) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor; 555 phalloidin (red).)
Testing Data
(Image: TCF4 (C9B9) staining of HCT-116 cells (left) or SHSY-5Y cells (right) using TCF4 (C9B9) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor; 555 phalloidin (red).)
Testing Data
(Image: Western blot analysis of total cell lysates from HCT116, DLD1 and HepG2 cells using TCF4 (C9B9) Rabbit mAb.)
Testing Data
(Image: Western blot analysis of total cell lysates from HCT116, DLD1 and HepG2 cells using TCF4 (C9B9) Rabbit mAb.)
TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting studies indicate that TCF4 is required to maintain the crypt stem cells of the small intestine (6,7). TCF4 has several splicing isoforms that are expressed differentially in tissues and during cancer progression (8,9). Studies also indicate that a variant of the TCF4 gene confers an increased risk of type 2 diabetes (10).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Aug 2011]
Uniprot Description
TCF4: Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'. Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin. Interacts with HIVEP2. Interacts with NEUROD2. Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 18q21.1
Cellular Component: transcription factor complex; nucleus
Molecular Function: protein C-terminus binding; protein binding; DNA binding; protein heterodimerization activity; transcription factor activity
Biological Process: transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; transcription initiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; protein-DNA complex assembly
Disease: Corneal Dystrophy, Fuchs Endothelial, 3; Pitt-hopkins Syndrome
Research Articles on TCF4
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Product Notes
The TCF4 tcf4 (Catalog #AAA555277) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The TCF4 (C9B9), Monoclonal Antibody, Rabbit reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TCF4 (C9B9) can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the TCF4 tcf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCF4 (C9B9), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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