Typical Testing Data/Standard Curve
Rat Transcription factor 4 (TCF4) ELISA Kit | TCF4 elisa kit
Rat Transcription factor 4 (TCF4) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||TCF4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TCF4 antibody and an TCF4-HRP conjugate. The assay sample and buffer are incubated together with TCF4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TCF4 concentration since TCF4 from samples and TCF4-HRP conjugate compete for the anti-TCF4 antibody binding site. Since the number of sites is limited, as more sites are occupied by TCF4 from the sample, fewer sites are left to bind TCF4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TCF4 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Aug 2011]
Uniprot Description
TCF4: Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'. Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin. Interacts with HIVEP2. Interacts with NEUROD2. Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 18q21.1
Cellular Component: transcription factor complex; nucleus
Molecular Function: protein C-terminus binding; protein binding; DNA binding; protein heterodimerization activity; transcription factor activity
Biological Process: transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; transcription initiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; protein-DNA complex assembly
Disease: Corneal Dystrophy, Fuchs Endothelial, 3; Pitt-hopkins Syndrome
Research Articles on TCF4
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Product Notes
The Rat TCF4 tcf4 (Catalog #AAA7211727) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7211727 ELISA Kit recognizes Rat TCF4. It is sometimes possible for the material contained within the vial of "Transcription factor 4 (TCF4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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