Interleukin-17A Recombinant Protein | IL-17 recombinant protein

Human Interleukin-17A Recombinant

Cat. Num. AAA553185

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Interleukin-17A; Human Interleukin-17A Recombinant; TRF; il17; il-17; interleukin-17; IL-17 recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Human Interleukin-17 was lyophilized from a 0.2 um filtered PBS solution pH 7.5.
• Sequence: MRMLLHLSLL ALGAAYVYAI PTEIPTSALV KETLALLSTH RTLLIANETL RIPVPVHKNH QLCTEEIFQG IGTLESQTVQ GGTVERLFKN LSLIKKYIDG QKKKCGEERR RVNQFLDYLQ EFLGVMNTEW IIES
• Sequence Length: 155

• Domain: four helix cytokine core
• Host Note: Optimized DNA sequence encoding Human Interleukin-17A mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human Interleukin17A, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 16kDa.; ; Recombinant IL-17A is a disulfide-linked homodimeric protein consisting of two 137 amino acid residue subunits, and migrates as an approximately 31kDa protein under non-reducing conditions and as a 16 kDa protein under reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IL-17 recombinant protein

Interleukin-17 (IL-17 or IL- 17A) is the founder member of the IL-17 cytokine family, which shares 50% homology with IL-17F. IL-17 cytokines are produced by T- helper (TH) 17 cells, γδ T cells, and other immune cells. IL-17A and IL-17F form homodimers and heterodimers as functional ligands. IL-17 cytokines bind to 2 receptors, IL-17RA and IL-17RC. IL-17RC forms a receptor heterodimer with IL-17RA. The receptor heterodimer is preferred by IL-17 ligands, thus, either IL-17RA knockout (KO) or IL- 17RC KO completely abolishes IL-17 signaling. IL-17A and IL-17F are key proinflammatory cytokines involved in many inflammatory and autoimmune diseases.

Product Categories/Family for IL-17 recombinant protein

Cytokines

References

• the innate immune response to uropathogenic escherichia coli involves IL-17a in a murine model of urinary tract infection .J. Immunol., Jan 2010; 10.4049/jimmunol.0902386.
• characterization of IL-17a–producing cells in celiac disease mucosa .J. Immunol., Jan 2010; 10.4049/jimmunol.0901919.
• enhanced production of IL-17a during zymosan-induced peritonitis in obese mice .J. Leukoc. Biol., Jan 2010; 87: 51 - 58.
• IL-17a and il-17f stimulate chemokines via mapk pathways (erk1/2 and p38 but not jnk) in mouse cultured mesangial cells: synergy with tnf- and il-1 .Am J Physiol Renal Physiol, Dec 2009; 10.1152/ajprenal.00198.2009.
• engineering of stable bispecific antibodies targeting IL-17a and il-23 .Protein Eng. Des. Sel., Dec 2009; 10.1093/protein/gzp073.