FLT3 Ligand Recombinant Protein | FLT3 recombinant protein

Mouse FLT3 Ligand Recombinant

Cat. Num. AAA553017

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: FLT3 Ligand; Mouse FLT3 Ligand Recombinant; flt3 ligand; flt3lg; SL cytokine; Fms; FLT3 recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant FLT-3 Ligand was lyophilized from a 0.2 um filtered solution with 50mM Tris,100mM NaCl, pH 8.5.
• Sequence: MTVLAPAWSP NSSLLLLLLL LSPCLRGTPD CYFSHSPISS NFKVKFRELT DHLLKDYPVT VAVNLQDEKH CKALWSLFLA QRWIEQLKTV AGSKMQTLLE DVNTEIHFVT SCTFQPLPEC LRFVQTNISH LLKDTCTQLL ALKPCIGKAC QNFSRCLEVQ CQPDSSTLLP PRSPIALEAT ELPEPRPRQL LLLLLLLLPL TLVLLAAAWG LRWQRARRRG ELHPGVPLPS HP
• Sequence Length: 232

• Domain: four helix cytokine core
• Host Note: Optimized DNA sequence encoding mouse FLT-3 ligand mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native mouse FLT-3 Ligand, generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated molecular mass of approximately 19 kDa.; ; Recombinant FLT-3 Ligand is a disulfide-linked monomer protein consisting of 164 amino acid residue subunits, and migrates as an approximately 32 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for FLT3 recombinant protein

FLT-3 ligand promotes long- term expansion and differentiation of human pro-B-cells in the presence of IL7 or in combination of IL7 and IL3.The human flt-3 ligand also stimulates the proliferation of cells expressing murine flt-3 receptors.n combination with SCF and IL3 flt-3 ligand can cause expansion of cells with the marker spectrum CD34 (+) CD38 (-). Alone, flt- 3 ligand supports the survival of precursors in the lineage of blood-forming cells such as CFU-GM, CFU- GEMM, and the very primitive high proliferative potential colony-forming cells, HPP- CFC. flt-3 ligand only has marginal effects on progenitors for erythroid cells and megakaryocytes.

Product Categories/Family for FLT3 recombinant protein

Cytokines

References

• tumor vaccines expressing FLT3 Ligand synergize with ctla-4 blockade to reject preimplanted tumors .Cancer Res., Sep 2009; 10.1158/0008-5472.CAN-08-3289.
• the concerted action of gm-csf and flt3-ligand on in vivo dendritic cell homeostasis .Blood, Jul 2009; 114: 835 - 843.
• ectodomain shedding of FLT3 Ligand is mediated by tnf- converting enzyme .J. Immunol., Jun 2009; 182: 7408 - 7414.
• transplanted cd34+ cells stimulated with FLT3 Ligand and granulocyte-colony stimulating factor display an impaired early reconstitution capacity .Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 4591.
• hematopoietic stem - and progenitor cells are differentially mobilized in response to flt3-ligand .Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 4782.