Trefoil Factor 1 Active Protein | TFF1 active protein

Recombinant Human Trefoil Factor 1 (rHu TFF-1)

Cat. Num. AAA546085

• Gene Names: TFF1; pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
• Purity: >97% by SDS-PAGE and HPLC analyses.
• Synonyms: Trefoil Factor 1; Recombinant Human Trefoil Factor 1 (rHu TFF-1); human TFF1; Human Trefoil Factor 1; TFF1 active protein

• Host: E Coli
• Purity/Purification: >97% by SDS-PAGE and HPLC analyses.
• Form/Format: Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.; Physical Appearance: Sterile FilteredWhite lyophilized (freeze-dried) powder.
• Sequence: EAQTETCTVA PRERQNCGFP GVTPSQCANK GCCFDDTVRG VPWCFYPNTI DVPPEEECEF
• Sequence Length: 84

• Molecular Weight Information: 13.2 kDa, a single, non-glycosylated polypeptide chain containing 111 amino acids.
• Biological Activity: Fully biologically active when compared to standard.Determined by its ability to activate ERK1/2 (a MAPkinase signaling molecule) using a concentration 1.0-2.0 ug/ml.
• Endotoxin Level: Less than 1EU/mg of rHuTFF1 as determined by LAL method.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions.
• Preparation and Storage: This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.

Related Product Information for TFF1 active protein

The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF1 is essential for normal differentiation of the antral and pyloric gastric mucosa and functions as a gastric-specific tumor suppressor gene.

NCBI and Uniprot Product Information

• NCBI GI #: 4507451
• NCBI GeneID: 7031
• NCBI Accession #: NP_003216.1
• NCBI GenBank Nucleotide #: NM_003225.2
• UniProt Accession #: P04155
• NCBI Official Full Name: trefoil factor 1
• NCBI Official Synonym Full Names: trefoil factor 1
• NCBI Official Symbol: TFF1
• NCBI Official Synonym Symbols: pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
• NCBI Protein Information: trefoil factor 1; protein pS2; polypeptide P1.A; gastrointestinal trefoil protein pS2; breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence
• UniProt Protein Name: Trefoil factor 1
• Protein Family: Trefoil factor
• UniProt Gene Name: TFF1
• UniProt Synonym Gene Names: BCEI; PS2; hP1.A
• UniProt Entry Name: TFF1_HUMAN

NCBI Description

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

Uniprot Description

TFF1: Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular space

Molecular Function: protein binding; growth factor activity

Biological Process: response to peptide hormone stimulus; negative regulation of cell proliferation; maintenance of gastrointestinal epithelium; carbohydrate metabolic process; digestion; cell differentiation; response to iron ion; response to estradiol stimulus