Bovine Apolipoprotein L1 ELISA Kit | APOL1 elisa kit

Bovine Apolipoprotein L1 ELISA Kit

Cat. Num. AAA742366

• Gene Names: APOL1; APOL; APO-L; FSGS4; APOL-I
• Reactivity: Bovine
• Synonyms: Apolipoprotein L1; Bovine Apolipoprotein L1 ELISA Kit; APOL1 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This APOL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine APOL1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOL1 elisa kit

Principle of the Assay: APOL1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for APOL1. Standards or samples are then added to the microtiter plate wells and APOL1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of APOL1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for APOL1 are added to each well to "sandwich" the APOL1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain APOL1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APOL1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for APOL1 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 211938440
• NCBI GeneID: 8542
• NCBI Accession #: NP_001130012.1
• NCBI GenBank Nucleotide #: NM_001136540.1
• Molecular Weight: 42,158 Da
• NCBI Official Full Name: apolipoprotein L1 isoform a
• NCBI Official Synonym Full Names: apolipoprotein L, 1
• NCBI Official Symbol: APOL1
• NCBI Official Synonym Symbols: APOL; APO-L; FSGS4; APOL-I
• NCBI Protein Information: apolipoprotein L1
• UniProt Protein Name: Apolipoprotein L1
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOL1
• UniProt Synonym Gene Names: APOL; Apo-L; ApoL; ApoL-I
• UniProt Entry Name: APOL1_HUMAN

NCBI Description

This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]

Uniprot Description

APOL1: May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4). It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Belongs to the apolipoprotein L family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Channel, chloride; Secreted, signal peptide; Lipid-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular space; extracellular region; intrinsic to membrane

Molecular Function: protein binding; chloride channel activity; lipid binding

Biological Process: cholesterol metabolic process; receptor-mediated endocytosis; killing of cells of another organism; cytolysis; innate immune response; chloride transport; lipoprotein metabolic process; lipid transport

Disease: Focal Segmental Glomerulosclerosis 4, Susceptibility To