Western Blot
(Western Blot analysis using DYKDDDDK antibody1:1000 (1 ug/ml) antibody dilution probed against HEK 293 cells transfected with DYKDDDDK-tagged protein vector; unstranfected (1) and transfected (2). )
Mouse DYKDDDDK Monoclonal Antibody | anti-DYKDDDDK antibody
DYKDDDDK antibody
Applications
Dot Blot, ELISA, Immunoprecipitation, Western Blot
Purity
Method of purificaiton: DYKDDDDK antibody was purifies by Protein A chromatography.
Synonyms
DYKDDDDK; Monoclonal Antibody; DYKDDDDK antibody; Monoclonal DYKDDDDK; Anti DYKDDDDK; DYKDDDDK tag; FG4R; anti-DYKDDDDK antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
FG4R
Specificity
DYKDDDDK tag
Purity/Purification
Method of purificaiton: DYKDDDDK antibody was purifies by Protein A chromatography.
Form/Format
Supplied in 10mM PBS, pH 7.2.
Concentration
1.5 mg/ml (varies by lot)
Sequence Length
123
Applicable Applications for anti-DYKDDDDK antibody
Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
WB: 1:1000-3000
Cross-Reactivity
Recognizes the N-terminal , C-terminal or internal DYKDDDDK-tagged fusion proteins.
Immunogen
DYKDDDDK (DYKDDDDK) synthetic peptide conjugated to KLH.
Preparation and Storage
Store at 4°C for short term, store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Western Blot
(Western Blot analysis using DYKDDDDK antibody1:1000 (1 ug/ml) antibody dilution probed against HEK 293 cells transfected with DYKDDDDK-tagged protein vector; unstranfected (1) and transfected (2). )
Western Blot
(Western Blot analysis using DYKDDDDK antibody1:1000 (1 ug/ml) antibody dilution probed against HEK 293 cells transfected with DYKDDDDK-tagged protein vector; unstranfected (1) and transfected (2). )
Related Product Information for anti-DYKDDDDK antibody
Biological Significance: DYKDDDDK tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A DYKDDDDK-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a DYKDDDDK-tag to this protein allows one to follow the protein with an antibody against the DYKDDDDK sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.!!Cross Reactivity||Recognize the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
DYKDDDDK
UniProt Protein Name
DYKDDDDK
UniProt Gene Name
DYKDDDDK
UniProt Entry Name
G8Q0L4_PSEFL
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Product Notes
The DYKDDDDK dykddddk (Catalog #AAA533391) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DYKDDDDK can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB). WB: 1:1000-3000. Researchers should empirically determine the suitability of the DYKDDDDK dykddddk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DYKDDDDK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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