Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using ROMK/Kir1.1 (Phospho-Ser44/25) antibody.)
Rabbit ROMK Polyclonal Antibody | anti-ROMK antibody
ROMK antibody (Ser44/25)
Applications
Immunohistochemistry, Immunofluorescence, ELISA
Purity
ROMK antibody was purified by affinity chromatography
Synonyms
ROMK; Polyclonal Antibody; ROMK antibody (Ser44/25); Polyclonal ROMK; Anti-ROMK; ATP-regulated potassium channel ROM-K; ATP-sensitive inward rectifier potassium channel 1; Inward rectifier K(+) channel Kir1.1; inwardly rectifying K+ channel; IRK1; KCNJ1; KIR1.1; Potassium channel inwardly rectifying subfamily J member 1; potassium inwardly-rectifying channel J1; potassium inwardly-rectifying channel subfamily J member 1; ROMK1; anti-ROMK antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
ROMK antibody (Ser44/25) detects endogenous levels of ROMK only when phosphorylated at serine 44/25
Purity/Purification
ROMK antibody was purified by affinity chromatography
Form/Format
Supplied in liquid format, PBS buffer, pH 7.4, 150mM NaCl with added preservative 0.02% sodium azide and 50% glycerol
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-ROMK antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
IHC: 1:50 - 1:100
IF: 1:100 - 1:500
ELISA: 1:5000
IF: 1:100 - 1:500
ELISA: 1:5000
Biological Significance
ROMK is an inward rectifier-type potassium channel protein. Probably plays a major role in potassium homeostasis in the kidney.
Cross-Reactivity
Mouse, Rat - Reactive with proteins phosphorylated as follows: Human: Ser44, Mouse: Ser25, Rat: Ser44
Immunogen
ROMK antibody was raised in Rabbit using a synthesized phosphopeptide derived from human ROMK around the phosphorylation site of serine 44/25 (L-V-SP-K-D) as the immunogen
Preparation and Storage
Aliquot and store at -20 degree C. Avoid repeated freeze thaw cycles
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using ROMK/Kir1.1 (Phospho-Ser44/25) antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using ROMK/Kir1.1 (Phospho-Ser44/25) antibody.)
Related Product Information for anti-ROMK antibody
Rabbit polyclonal ROMK antibody (Ser44/25)
Product Categories/Family for anti-ROMK antibody
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Product Notes
The ROMK (Catalog #AAA5300104) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ROMK can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). IHC: 1:50 - 1:100 IF: 1:100 - 1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the ROMK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ROMK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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