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Testing Data (P-peptide-+)

ROMK/Kir1.1 Antibody | anti-ROMK/Kir1.1 antibody

ROMK/Kir1.1 (Phospho-Ser44/25)Antibody

Gene Names
KCNJ1; ROMK; ROMK1; KIR1.1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
ROMK/Kir1.1; ROMK/Kir1.1 (Phospho-Ser44/25)Antibody; ATP-regulated potassium channel ROM-K; ATP-sensitive inward rectifier potassium channel 1; IRK1; KAB-1; KCNJ1; Kir1.1; Potassium channel; inwardly rectifying; subfamily J; member 1; ROMK1; anti-ROMK/Kir1.1 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
ROMK/Kir1.1 (Phospho-Ser44/25) antibody detects endogenous levels of ROMK/Kir1.1 only when phosphorylated at serine 44/25.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
391
Applicable Applications for anti-ROMK/Kir1.1 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human ROMK/Kir1.1 around the phosphorylation site of serine 44/25 (L-V-SP-K-D).
All Sites
Human: Ser44
Mouse: Ser25
Rat: Ser44
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

(P-peptide-+)

Testing Data (P-peptide-+)

Immunohistochemistry (IHC)

(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using ROMK/Kir1.1 (Phospho-Ser44/25) antibody. Immunofluorescence analysis of A549 cells, using ROMK/Kir1.1 (Phospho-Ser44/25) antibody.)

Immunohistochemistry (IHC) (P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using ROMK/Kir1.1 (Phospho-Ser44/25) antibody. Immunofluorescence analysis of A549 cells, using ROMK/Kir1.1 (Phospho-Ser44/25) antibody.)

Immunofluorescence (IF)

(ROMK/Kir1.1 (Phospho-Ser44/25) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Immunofluorescence (IF) (ROMK/Kir1.1 (Phospho-Ser44/25) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
References
DW Lundgren, Proc. Soc. Exp. Biol. Med., Oct 1997; 216: 57. Dana Yoo, J. Biol. Chem., Jun 2003; 278: 23066-23075. W. S. Lee, Am J Physiol Renal Physiol, Jun 1995; 268: 1124.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,611 Da
NCBI Official Full Name
ATP-sensitive inward rectifier potassium channel 1 isoform a
NCBI Official Synonym Full Names
potassium voltage-gated channel subfamily J member 1
NCBI Official Symbol
KCNJ1
NCBI Official Synonym Symbols
ROMK; ROMK1; KIR1.1
NCBI Protein Information
ATP-sensitive inward rectifier potassium channel 1
UniProt Protein Name
ATP-sensitive inward rectifier potassium channel 1
UniProt Gene Name
KCNJ1
UniProt Synonym Gene Names
ROMK1

NCBI Description

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium.

Research Articles on ROMK/Kir1.1

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Product Notes

The ROMK/Kir1.1 kcnj1 (Catalog #AAA8509781) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The ROMK/Kir1.1 (Phospho-Ser44/25)Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ROMK/Kir1.1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). IHC: 1:50-1:100 IF: 1:100-1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the ROMK/Kir1.1 kcnj1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ROMK/Kir1.1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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