Rat anti-Mouse CD8a Monoclonal Antibody | anti-CD8a antibody

Anti-Mouse CD8a (Ly 2), APC (Clone CT-CD8a)(rat IgG2a)

Cat. Num. AAA524523

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunohistochemistry
• Synonyms: CD8a; Monoclonal Antibody; Anti-Mouse CD8a (Ly 2); APC (Clone CT-CD8a)(rat IgG2a); anti-CD8a antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Clone Number: CT-CD8a
• Specificity: Mouse CD8a (Ly 2; Lyt 2)
• Form/Format: APC conjugated IgG buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5mg/ml.

• Applicable Applications for anti-CD8a antibody: Flow Cytometry (FC/FACS), Vivo Assay, Immunohistochemistry-Frozen (IHC-F), Immunohistochemistry-Paraffin (IHC-P)
• Conjugation: Allophycocyanin (APC)
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE

Flow Cytometry (FC/FACS)

(Balb/c peritoneal macrophages (left) or bone marrow (Gr-1 gate)(right) were stained with anti-C5a receptor (CD88)(clone: 20/70)(filled histogram) or rat IgG2b isotype control (open histogram).)

Testing Data

(CL7588F (CD88 FITC) and CL8991PE (Gr-1 PE) staining on C57BL/6 mouse bone marrow.)

Related Product Information for anti-CD8a antibody

anti-mouse CD8a antigen monoclonal antibody binds to a protein of approximately 30,000 daltons found on 85% of mouse thymocytes, mouse cytotoxic/suppressor T cells and their precursors. It does not bind to mouse helper/inducer T cells. This antibody is not strain specific and can be used with all commonly used laboratory mice.

References

1. Tomonari, K. and Spencer, S. 1990 Epitope-specific binding of CD8 regulates activation of T cells and induction of cytotoxicity. International Immunolgy 2(12):1189-1194.
2. Whiteland, Joanne L., et al. 1995 Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. Journal of Histochemistry & Cytochemistry 43.3: 313-320.