IL28A recombinant protein

IL28A

Cat. Num. AAA517123

• Gene Names: IFNL2; IL28A; IL-28A
• Applications: Cell Assay
• Synonyms: IL28A; Zcyto20; Interleukin-28A; Interferon lambda-2; IL28A recombinant protein

• Host: E Coli
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS

• Applicable Applications for IL28A recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method
• Sample Cell Assay Data: The ED50 is 10.0-50.0ng/mL as determined by an anti-viral assay (using human hepatocellular carcinoma cells infected with EMC virus)
• Reconstitution: Reconstitute in sterile PBS at 100ug/mL (containing at least 0.1% human or bovine serum albumin)
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

SDS-Page

(Purity: Recombinant Human IL17D resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250)

Related Product Information for IL28A recombinant protein

Recombinant Human IL28A was expressed in E Coli cells. The protein accession number is Q8IZJ0
Interferon lambda-2, also known as Interleukin 28A, is an antiviral cytokine with immunomodulatory activity and is distantly related to the IL-10 family and type I interferons. IL28A along with IL29 and IL28B, are three closely related cytokines that form a cytokine gene cluster that use a distinct receptor system which induces antiviral defence signals. IL28A interacts with a heterodimeric class II cytokine receptor ligand and leads to the activation of the JAK/STAT signalling pathway, which ultimately leads to IFN-stimulated gene (ISG) and MHC class I antigen expression. It also plays a key role in antiviral defence in Toll-like receptors (TLR) and intestinal epithelia.

Product Categories/Family for IL28A recombinant protein

Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 26024321
• NCBI GeneID: 282616
• NCBI Accession #: NP_742150.1
• NCBI GenBank Nucleotide #: NM_172138.1
• UniProt Accession #: Q8IZJ0; Q45KQ8; Q6VN55; Q8IWL7
• Molecular Weight: 22,288 Da
• NCBI Official Full Name: interferon lambda-2
• NCBI Official Synonym Full Names: interferon, lambda 2
• NCBI Official Symbol: IFNL2
• NCBI Official Synonym Symbols: IL28A; IL-28A
• NCBI Protein Information: interferon lambda-2; IFN-lambda-2; interleukin-28A; cytokine Zcyto20; interferon lambda 2; interferon-lambda 2; interleukin 28A (interferon, lambda 2)
• UniProt Protein Name: Interferon lambda-2
• UniProt Gene Name: IFNL2
• UniProt Synonym Gene Names: IL28A; ZCYTO20; IFN-lambda-2; IL-28A
• UniProt Entry Name: IFNL2_HUMAN

NCBI Description

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B (IL28B), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

Uniprot Description

IL28A: Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 19q13.13

Cellular Component: extracellular space

Molecular Function: cytokine activity

Biological Process: positive regulation of immune response; mucosal immune response; JAK-STAT cascade; defense response to virus