Chicken Platelet Derived Growth Factor Receptor Like Protein ELISA Kit | PDGFRL elisa kit

Chicken Platelet Derived Growth Factor Receptor Like Protein ELISA Kit

Cat. Num. AAA734507

• Gene Names: PDGFRL; PDGRL; PRLTS
• Reactivity: Chicken
• Synonyms: Platelet Derived Growth Factor Receptor Like Protein; Chicken Platelet Derived Growth Factor Receptor Like Protein ELISA Kit; PDGFRL elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This PDGFRL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken PDGFRL. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDGFRL elisa kit

Principle of the Assay: PDGFRL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PDGFRL antibody and an PDGFRL-HRP conjugate. The assay sample and buffer are incubated together with PDGFRL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDGFRL concentration since PDGFRL from samples and PDGFRL-HRP conjugate compete for the anti-PDGFRL antibody binding site. Since the number of sites is limited, as more sites are occupied by PDGFRL from the sample, fewer sites are left to bind PDGFRL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDGFRL concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PDGFRL elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 5453872
• NCBI GeneID: 5157
• NCBI Accession #: NP_006198.1
• NCBI GenBank Nucleotide #: NM_006207.2
• UniProt Accession #: Q15198; Q6FH04; A8K085
• Molecular Weight: 41,861 Da
• NCBI Official Full Name: platelet-derived growth factor receptor-like protein
• NCBI Official Synonym Full Names: platelet-derived growth factor receptor-like
• NCBI Official Symbol: PDGFRL
• NCBI Official Synonym Symbols: PDGRL; PRLTS
• NCBI Protein Information: platelet-derived growth factor receptor-like protein; PDGFR-like protein; PDGF receptor beta-like tumor suppressor; platelet-derived growth factor-beta-like tumor suppressor
• UniProt Protein Name: Platelet-derived growth factor receptor-like protein
• Protein Family: Platelet-derived growth factor receptor-like protein
• UniProt Gene Name: PDGFRL
• UniProt Synonym Gene Names: PRLTS; PDGFR-like protein
• UniProt Entry Name: PGFRL_HUMAN

NCBI Description

This gene encodes a protein with significant sequence similarity to the ligand binding domain of platelet-derived growth factor receptor beta. Mutations in this gene, or deletion of a chromosomal segment containing this gene, are associated with sporadic hepatocellular carcinomas, colorectal cancers, and non-small cell lung cancers. This suggests this gene product may function as a tumor suppressor. [provided by RefSeq, Jul 2008]

Uniprot Description

PDGFRL: Defects in PDGFRL are associated with colorectal cancer (CRC).

Protein type: Receptor, misc.; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p22-p21.3

Cellular Component: extracellular region

Molecular Function: platelet activating factor receptor activity; platelet-derived growth factor beta-receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway

Disease: Hepatocellular Carcinoma